Protection in lambs vaccinated with Hamemonchus contortus antigens is age related, and correlates with IgE rather that IgG1 antibody

FNJ Kooyman*, HDFH Schallig, MAW Van Leeuwen, A Mackellar, JF Huntley, AWCA Cornelissen, L Vervelde

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Protection by vaccination with excretory-secretory products (ES) from Haemonchus contortus, containing predominantly proteins of 15 and 24 kDa, against an experimental challenge infection depends on the age of the sheep. Vaccinated sheep 9, 6 or 3 months of age were protected for 83%, 77% and -34%, respectively. There was a significant difference in ES-specific serum IgE response but not in IgG1 response, after the last vaccination between the different age groups. In the protected 9-month-old animals, there was an increase up to 18 times the prevaccination levels, while the increase in the unprotected 3-month-old animals was at most 1-4 times. The 6-month-old animals showed an intermediate increase of approximately six times the prevaccination level. There was no correlation within the 9-month-old sheep between ES-specific IgE levels and protection, measured as worm burden. However, when the different age groups were combined, there was a positive correlation (r=0.38) between protection and ES-specific IgE levels 1 week after the vaccination. At the end of the experiment, peripheral blood eosinophils and mast cell counts in abomasal tissue were also significantly higher in the vaccinated and challenged 9-month-old sheep than in the vaccinated and challenged 3-month-old or than in the 9-month-old sheep with challenge, but without vaccination. Increased serum IgE levels, eosinophilia and mucosal mast cell hyperplasia are the hallmarks of a Th2 response and were all demonstrated in protected, older sheep, but not in unprotected, younger sheep.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalParasite Immunology
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 2000

Keywords

  • IMMUNITY
  • BLOOD
  • ovine
  • SERUM IGE
  • IgE
  • HAEMONCHUS-CONTORTUS
  • EXCRETORY-SECRETORY PROTEINS
  • RESPONSES
  • Haemonchus contortus
  • vaccine
  • CELL
  • nematode
  • ES
  • IRRADIATED LARVAE
  • INFECTION
  • immune response
  • SHEEP

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