Protein crystallization: scaling of charge and salt concentration in lysozyme solutions

Wilson Poon; S U  Egelhaaf; P A  Beales; A  Salonen; L  Sawyer

Protein crystallization: scaling of charge and salt concentration in lysozyme solutions

Wilson Poon, S U Egelhaaf, P A Beales, A Salonen, L Sawyer

Research output: Contribution to journal › Letter › peer-review

Abstract

We studied the crystallization of lysozyme solutions by adding sodium chloride at pH = 4.5, 5.9 and 7.8. A universal crystallization boundary is found if data are scaled according to the salt concentration normalized by the square of the charge at the appropriate pH. Calculations show that this finding is consistent with recent attempts to rationalize protein crystallization using second virial coefficients.

Original language	English
Pages (from-to)	L569-L574
Number of pages	6
Journal	Journal of Physics: Condensed Matter
Volume	12
Issue number	35
Publication status	Published - 4 Sept 2000

Keywords / Materials (for Non-textual outputs)

SCATTERING
LIGHT

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title = "Protein crystallization: scaling of charge and salt concentration in lysozyme solutions",

abstract = "We studied the crystallization of lysozyme solutions by adding sodium chloride at pH = 4.5, 5.9 and 7.8. A universal crystallization boundary is found if data are scaled according to the salt concentration normalized by the square of the charge at the appropriate pH. Calculations show that this finding is consistent with recent attempts to rationalize protein crystallization using second virial coefficients.",

keywords = "SCATTERING, LIGHT",

author = "Wilson Poon and Egelhaaf, {S U} and Beales, {P A} and A Salonen and L Sawyer",

year = "2000",

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TY - JOUR

T1 - Protein crystallization: scaling of charge and salt concentration in lysozyme solutions

AU - Poon, Wilson

AU - Egelhaaf, S U

AU - Beales, P A

AU - Salonen, A

AU - Sawyer, L

PY - 2000/9/4

Y1 - 2000/9/4

N2 - We studied the crystallization of lysozyme solutions by adding sodium chloride at pH = 4.5, 5.9 and 7.8. A universal crystallization boundary is found if data are scaled according to the salt concentration normalized by the square of the charge at the appropriate pH. Calculations show that this finding is consistent with recent attempts to rationalize protein crystallization using second virial coefficients.

AB - We studied the crystallization of lysozyme solutions by adding sodium chloride at pH = 4.5, 5.9 and 7.8. A universal crystallization boundary is found if data are scaled according to the salt concentration normalized by the square of the charge at the appropriate pH. Calculations show that this finding is consistent with recent attempts to rationalize protein crystallization using second virial coefficients.

KW - SCATTERING

KW - LIGHT

M3 - Letter

SN - 0953-8984

VL - 12

SP - L569-L574

JO - Journal of Physics: Condensed Matter

JF - Journal of Physics: Condensed Matter

IS - 35

ER -

Protein crystallization: scaling of charge and salt concentration in lysozyme solutions

Abstract

Keywords / Materials (for Non-textual outputs)

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