Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Chiara Martino, Shin-Hyun Kim, Louise Horsfall, Alireza Abbaspourrad, Susan J. Rosser, Jonathan Cooper, David A. Weitz

Research output: Contribution to journalArticlepeer-review

Abstract

Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.
Original languageEnglish
Pages (from-to)6416-6420
Number of pages5
JournalAngewandte Chemie International Edition
Volume51
Issue number26
DOIs
Publication statusPublished - Jun 2012

Keywords

  • double emulsions
  • MreB
  • polymersomes
  • protein expression
  • synthetic biology

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