Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Chiara Martino; Shin-Hyun Kim; Louise Horsfall; Alireza Abbaspourrad; Susan J. Rosser; Jonathan Cooper; David A. Weitz

doi:10.1002/anie.201201443

Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Chiara Martino, Shin-Hyun Kim, Louise Horsfall, Alireza Abbaspourrad, Susan J. Rosser, Jonathan Cooper, David A. Weitz

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.

Original language	English
Pages (from-to)	6416-6420
Number of pages	5
Journal	Angewandte Chemie International Edition
Volume	51
Issue number	26
DOIs	https://doi.org/10.1002/anie.201201443
Publication status	Published - Jun 2012

Keywords / Materials (for Non-textual outputs)

double emulsions
MreB
polymersomes
protein expression
synthetic biology

Access to Document

10.1002/anie.201201443

http://onlinelibrary.wiley.com/doi/10.1002/anie.201201443/abstract

Cite this

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title = "Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures",

abstract = "Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.",

keywords = "double emulsions , MreB , polymersomes , protein expression, synthetic biology",

author = "Chiara Martino and Shin-Hyun Kim and Louise Horsfall and Alireza Abbaspourrad and Rosser, {Susan J.} and Jonathan Cooper and Weitz, {David A.}",

year = "2012",

month = jun,

doi = "10.1002/anie.201201443",

language = "English",

volume = "51",

pages = "6416--6420",

journal = "Angewandte Chemie International Edition",

issn = "1433-7851",

publisher = "Wiley-VCH",

number = "26",

}

TY - JOUR

T1 - Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

AU - Martino, Chiara

AU - Kim, Shin-Hyun

AU - Horsfall, Louise

AU - Abbaspourrad, Alireza

AU - Rosser, Susan J.

AU - Cooper, Jonathan

AU - Weitz, David A.

PY - 2012/6

Y1 - 2012/6

N2 - Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.

AB - Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.

KW - double emulsions

KW - MreB

KW - polymersomes

KW - protein expression

KW - synthetic biology

U2 - 10.1002/anie.201201443

DO - 10.1002/anie.201201443

M3 - Article

SN - 1433-7851

VL - 51

SP - 6416

EP - 6420

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 26

ER -

Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Cite this