Protein phosphatase 1 beta is required far the maintenance of muscle attachments

S Raghavan, I Williams, H Aslam, D Thomas, B Szoor, G Morgan, S Gross, J Turner, J Fernandes, K VijayRaghavan, L Alphey

Research output: Contribution to journalArticlepeer-review

Abstract

Type 1 serine/threonine protein phosphatases (PP1) are important regulators of many cellular and developmental processes, including glycogen metabolism, muscle contraction, and the cell cycle [1-5], Drosophila and humans both have multiple genes encoding PP1 isoforms [3,6,7]; each has one beta and several alpha isoform genes (alpha(1), alpha(2), alpha(3) in flies, alpha and gamma in humans; mammalian PP1 beta is also known as PP1 delta), The alpha/beta subtype differences are highly conserved between flies and mammals [6], Though all these proteins are >85% identical to each other and have indistinguishable activities in vitro, we show here that the Drosophila beta isoform has a distinct biological role, We show that PP1 beta 9C corresponds to flapwing (flw), previously identified mutants of which are viable but flightless because of defects in indirect flight muscles (IFMs) [8], We have isolated a new, semi-lethal flw allele that shows a range of defects, especially in muscles, which break away from their attachment sites and degenerate.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalCurrent Biology
Volume10
Issue number5
DOIs
Publication statusPublished - 1 Mar 2000

Keywords

  • catalytic subunit
  • Drosophila-Melanogaster
  • Serine/Threonine phosphatases
  • gene
  • mutations
  • mitosis
  • myosin
  • isoforms
  • contains
  • flight

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