Protein Tertiary Structure by Crosslinking/Mass Spectrometry

Michael Schneider, Adam Belsom, Juri Rappsilber*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Observing the structures of proteins within the cell and tracking structural changes under different cellular conditions are the ultimate challenges for structural biology. This, however, requires an experimental technique that can generate sufficient data for structure determination and is applicable in the native environment of proteins. Crosslinking/mass spectrometry (CLMS) and protein structure determination have recently advanced to meet these requirements and crosslinking-driven de novo structure determination in native environments is now possible. In this opinion article, we highlight recent successes in the field of CLMS with protein structure modeling and challenges it still holds.

Original languageEnglish
Pages (from-to)157-169
Number of pages13
JournalTrends in biochemical sciences
Issue number3
Early online date31 Jan 2018
Publication statusPublished - 1 Mar 2018


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