Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection

Eleanor Watson*, M. Pilar Alberdi, Neil F. Inglis, Alex Lainson, Megan E. Porter, Erin Manson, Lisa Imrie, Kevin Mclean, David G. E. Smith

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Lawsonia intracellularis is the aetiological agent of the commercially significant porcine disease, proliferative enteropathy. Current understanding of host-pathogen interaction is limited due to the fastidious microaerophilic obligate intracellular nature of the bacterium. In the present study, expression of bacterial proteins during infection was investigated using a mass spectrometry approach. LC-ESI-MS/MS analysis of two isolates of L intracellularis from heavily-infected epithelial cell cultures and database mining using fully annotated L intracellutaris genome sequences identified 19 proteins. According to the Clusters of Orthologous Groups (COG) functional classification, proteins were identified with roles in cell metabolism, protein synthesis and oxidative stress protection; seven proteins with putative or unknown function were also identified. Detailed bioinformatic analyses of five uncharacterised proteins, which were expressed by both isolates, identified domains and motifs common to other outer membrane-associated proteins with important roles in pathogenesis including adherence and invasion. Analysis of recombinant proteins on Western blots using immune sera from L. intracellularis-infected pigs identified two proteins, LI0841 and LI0902 as antigenic. The detection of five outer membrane proteins expressed during infection, including two antigenic proteins, demonstrates the potential of this approach to interrogate L intracellularis host-pathogen interactions and identify novel targets which may be exploited in disease control. (C) 2014 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)448-455
Number of pages8
JournalVeterinary Microbiology
Issue number3-4
Publication statusPublished - 5 Dec 2014

Keywords / Materials (for Non-textual outputs)

  • Lawsonia intracellularis
  • Proliferative enteropathy
  • Shotgun proteomics
  • Surface proteins
  • Serology
  • GENE


Dive into the research topics of 'Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection'. Together they form a unique fingerprint.

Cite this