Proteomic analysis of pathogenic and attenuated alcelaphine herpesvirus 1

Inga Dry, David M Haig, Neil F Inglis, Lisa Imrie, James P. Stewart, George C Russell

Research output: Contribution to journalArticlepeer-review

Abstract

The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) causes malignant catarrhal fever in susceptible ungulates but infects its natural host, wildebeest, without obvious clinical signs. In tissue culture, AlHV-1 is initially predominantly cell associated and virulent but on extended culture becomes cell-free and attenuated. We wanted to determine what changes in protein composition had taken place during the transition from virulent to attenuated virus in culture. Purified virus preparations were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and proteins were analyzed by liquid chromatography-electrospray ionization-tandem mass spectrometry. Peptides were identified in serial gel slices by using MASCOT software to interrogate virus-specific and nonredundant sequence databases. Twenty-three AlHV-1-encoded proteins and six cellular proteins were identified in the attenuated and virulent viruses. Two polypeptides were detected in only the virulent virus preparations, while one other protein was found in only the attenuated virus. Two of these virus-specific proteins were identified by a single peptide, suggesting that these may be low-abundance virion proteins rather than markers of attenuation or pathogenesis. The results suggest that attenuation of AlHV-1 is not the result of gross changes in the composition of the virus particle but probably due to altered viral gene expression in the infected cell.
Original languageEnglish
Pages (from-to)5390-7
Number of pages8
JournalJournal of Virology
Volume82
Issue number11
Publication statusPublished - Jun 2008

Keywords

  • Animals
  • Capsid Proteins/chemistry
  • Capsid Proteins/metabolism
  • Cattle
  • Cell Line
  • Gammaherpesvirinae/chemistry
  • Gammaherpesvirinae/metabolism
  • Gammaherpesvirinae/pathogenicity
  • Glycoproteins/chemistry
  • Glycoproteins/metabolism
  • Mass Spectrometry
  • Proteomics
  • Rabbits
  • Receptors, Virus/metabolism
  • Sensitivity and Specificity
  • Viral Nonstructural Proteins/chemistry
  • Viral Nonstructural Proteins/metabolism
  • Virion/chemistry
  • Virion/metabolism

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