Proteomic analysis of postsynaptic proteins in regions of the human neocortex

Marcia Roy; Oksana Sorokina; Nathan Skene; Clémence Simonnet; Francesca Mazzola; Ruud Zwart; Emanuele Sher; Colin Smith; J. Douglas Armstrong; Seth Grant

doi:10.1038/s41593-017-0025-9

Proteomic analysis of postsynaptic proteins in regions of the human neocortex

Marcia Roy, Oksana Sorokina, Nathan Skene, Clémence Simonnet, Francesca Mazzola, Ruud Zwart, Emanuele Sher, Colin Smith, J. Douglas Armstrong, Seth Grant

Research output: Contribution to journal › Article › peer-review

Abstract

The postsynaptic proteome of excitatory synapses comprises ~1,000 highly conserved proteins that control the behavioral repertoire and mutations disrupting their function cause >130 brain diseases. Here, we document the composition of postsynaptic proteomes in human neocortical regions and integrate it with genetic, functional and structural magnetic resonance imaging, positron emission tomography imaging, and behavioral data. Neocortical regions show signatures of expression of individual proteins, protein complexes, biochemical and metabolic pathways. The compositional signatures in brain regions involved with language, emotion and memory functions were characterized. Integrating large-scale GWAS with regional proteome data identifies the same cortical region for smoking behavior as found with fMRI data. The neocortical postsynaptic proteome data resource can be used to link genetics to brain imaging and behavior, and to study the role of postsynaptic proteins in localization of brain functions.

Original language	English
Pages (from-to)	130–138
Number of pages	15
Journal	Nature Neuroscience
Volume	21
Issue number	1
Early online date	4 Dec 2017
DOIs	https://doi.org/10.1038/s41593-017-0025-9
Publication status	Published - 1 Jan 2018

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Proteomic analysis of postsynaptic proteins in regions of the human neocortex
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Cite this

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abstract = "The postsynaptic proteome of excitatory synapses comprises \textasciitilde{}1,000 highly conserved proteins that control the behavioral repertoire and mutations disrupting their function cause >130 brain diseases. Here, we document the composition of postsynaptic proteomes in human neocortical regions and integrate it with genetic, functional and structural magnetic resonance imaging, positron emission tomography imaging, and behavioral data. Neocortical regions show signatures of expression of individual proteins, protein complexes, biochemical and metabolic pathways. The compositional signatures in brain regions involved with language, emotion and memory functions were characterized. Integrating large-scale GWAS with regional proteome data identifies the same cortical region for smoking behavior as found with fMRI data. The neocortical postsynaptic proteome data resource can be used to link genetics to brain imaging and behavior, and to study the role of postsynaptic proteins in localization of brain functions.",

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AU - Roy, Marcia

AU - Sorokina, Oksana

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AU - Simonnet, Clémence

AU - Mazzola, Francesca

AU - Zwart, Ruud

AU - Sher, Emanuele

AU - Smith, Colin

AU - Armstrong, J. Douglas

AU - Grant, Seth

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AB - The postsynaptic proteome of excitatory synapses comprises ~1,000 highly conserved proteins that control the behavioral repertoire and mutations disrupting their function cause >130 brain diseases. Here, we document the composition of postsynaptic proteomes in human neocortical regions and integrate it with genetic, functional and structural magnetic resonance imaging, positron emission tomography imaging, and behavioral data. Neocortical regions show signatures of expression of individual proteins, protein complexes, biochemical and metabolic pathways. The compositional signatures in brain regions involved with language, emotion and memory functions were characterized. Integrating large-scale GWAS with regional proteome data identifies the same cortical region for smoking behavior as found with fMRI data. The neocortical postsynaptic proteome data resource can be used to link genetics to brain imaging and behavior, and to study the role of postsynaptic proteins in localization of brain functions.

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Proteomic analysis of postsynaptic proteins in regions of the human neocortex

Abstract

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