Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

Keith Sexton; Dominique Balharry; Paul Brennan; James McLaren; Ian A Brewis; Kelly A BéruBé

doi:10.3109/1354750X.2011.608855

Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

Keith Sexton, Dominique Balharry, Paul Brennan, James McLaren, Ian A Brewis, Kelly A BéruBé

Research output: Contribution to journal › Article › peer-review

Abstract

Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.

Original language	English
Pages (from-to)	567-76
Number of pages	10
Journal	Biomarkers
Volume	16
Issue number	7
DOIs	https://doi.org/10.3109/1354750X.2011.608855
Publication status	Published - 4 Oct 2011

Keywords

Biomarkers
Blotting, Western
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Gene Expression Profiling
Humans
Proteome
Proteomics
Respiratory Mucosa
Smoking
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Access to Document

10.3109/1354750X.2011.608855

Cite this

@article{3c6658318189418889e32e131693d7a2,

title = "Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components",

abstract = "Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.",

keywords = "Biomarkers, Blotting, Western, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Gene Expression Profiling, Humans, Proteome, Proteomics, Respiratory Mucosa, Smoking, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization",

author = "Keith Sexton and Dominique Balharry and Paul Brennan and James McLaren and Brewis, {Ian A} and B{\'e}ruB{\'e}, {Kelly A}",

year = "2011",

month = oct,

day = "4",

doi = "10.3109/1354750X.2011.608855",

language = "English",

volume = "16",

pages = "567--76",

journal = "Biomarkers",

issn = "1354-750X",

publisher = "Informa Healthcare",

number = "7",

}

TY - JOUR

T1 - Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

AU - Sexton, Keith

AU - Balharry, Dominique

AU - Brennan, Paul

AU - McLaren, James

AU - Brewis, Ian A

AU - BéruBé, Kelly A

PY - 2011/10/4

Y1 - 2011/10/4

N2 - Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.

AB - Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.

KW - Biomarkers

KW - Blotting, Western

KW - Cells, Cultured

KW - Electrophoresis, Polyacrylamide Gel

KW - Gene Expression Profiling

KW - Humans

KW - Proteome

KW - Proteomics

KW - Respiratory Mucosa

KW - Smoking

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

U2 - 10.3109/1354750X.2011.608855

DO - 10.3109/1354750X.2011.608855

M3 - Article

C2 - 21966894

VL - 16

SP - 567

EP - 576

JO - Biomarkers

JF - Biomarkers

SN - 1354-750X

IS - 7

ER -

Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

Abstract

Keywords

Access to Document

Fingerprint

Cite this