Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

Keith Sexton, Dominique Balharry, Paul Brennan, James McLaren, Ian A Brewis, Kelly A BéruBé

Research output: Contribution to journalArticlepeer-review

Abstract

Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.

Original languageEnglish
Pages (from-to)567-76
Number of pages10
JournalBiomarkers
Volume16
Issue number7
DOIs
Publication statusPublished - 4 Oct 2011

Keywords

  • Biomarkers
  • Blotting, Western
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Profiling
  • Humans
  • Proteome
  • Proteomics
  • Respiratory Mucosa
  • Smoking
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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