Proteomics and in silico approaches to extend understanding of the glutathione transferase superfamily of the tropical liver fluke Fasciola gigantica

Russell M Morphew, Neil Eccleston, Toby J Wilkinson, John McGarry, Samirah Perally, Mark Prescott, Deborah Ward, Diana Williams, Steve Paterson, M Raman

Research output: Contribution to journalArticlepeer-review

Abstract

Fasciolosis is an important foodborne, zoonotic disease of livestock and humans, with global annual health and economic losses estimated at several billion US$. Fasciola hepatica is the major species in temperate regions, while F. gigantica dominates in the tropics. In the absence of commercially available vaccines to control fasciolosis, increasing reports of resistance to current chemotherapeutic strategies and the spread of fasciolosis into new areas, new functional genomics approaches are being used to identify potential new drug targets and vaccine candidates. The glutathione transferase (GST) superfamily is both a candidate drug and vaccine target. This study reports the identification of a putatively novel Sigma class GST, present in a water-soluble cytosol extract from the tropical liver fluke F. gigantica. The GST was cloned and expressed as an enzymically active recombinant protein. This GST shares a greater identity with the human schistosomiasis GST vaccine currently at Phase II clinical trials than previously discovered F. gigantica GSTs, stimulating interest in its immuno-protective properties. In addition, in silico analysis of the GST superfamily of both F. gigantica and F. hepatica has revealed an additional Mu class GST, Omega class GSTs, and for the first time, a Zeta class member
Original languageEnglish
Pages (from-to)5876-5889
Number of pages14
JournalJournal Of Proteome Research
Volume11
Issue number12
DOIs
Publication statusPublished - 20 Nov 2012

Keywords

  • Fasciola gigantica;
  • Glutathione Transferase
  • Mu
  • Omega
  • Sigma
  • Zeta

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