Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining

Beatriz Maio; Nagore Elu; Cristina Martinez-Gonzalez; Emily K. Osterweil; Susana R. Louros

doi:10.1016/j.xpro.2024.103482

Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining

Beatriz Maio, Nagore Elu, Cristina Martinez-Gonzalez, Emily K. Osterweil^*, Susana R. Louros^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis. For complete details on the use and execution of this protocol, please refer to Louros et al.¹

Original language	English
Article number	103482
Number of pages	18
Journal	Star Protocols
Volume	5
Issue number	4
Early online date	11 Dec 2024
DOIs	https://doi.org/10.1016/j.xpro.2024.103482
Publication status	Published - 20 Dec 2024

Keywords / Materials (for Non-textual outputs)

microscopy
model organisms
neuroscience

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10.1016/j.xpro.2024.103482Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostainingFinal published version, 6.94 MBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

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title = "Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining",

abstract = "cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis. For complete details on the use and execution of this protocol, please refer to Louros et al.1",

keywords = "microscopy, model organisms, neuroscience",

author = "Beatriz Maio and Nagore Elu and Cristina Martinez-Gonzalez and Osterweil, \{Emily K.\} and Louros, \{Susana R.\}",

note = "S.R.L. and E.K.O. designed the experiments. B.M., N.E., C.M.-G., and S.R.L. conducted experiments. B.M., N.E., C.M.-G., S.R.L., and E.K.O. wrote and edited the manuscript.",

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AU - Maio, Beatriz

AU - Elu, Nagore

AU - Martinez-Gonzalez, Cristina

AU - Osterweil, Emily K.

AU - Louros, Susana R.

N1 - S.R.L. and E.K.O. designed the experiments. B.M., N.E., C.M.-G., and S.R.L. conducted experiments. B.M., N.E., C.M.-G., S.R.L., and E.K.O. wrote and edited the manuscript.

PY - 2024/12/20

Y1 - 2024/12/20

N2 - cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis. For complete details on the use and execution of this protocol, please refer to Louros et al.1

AB - cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis. For complete details on the use and execution of this protocol, please refer to Louros et al.1

KW - microscopy

KW - model organisms

KW - neuroscience

UR - https://www.scopus.com/pages/publications/85211490943

U2 - 10.1016/j.xpro.2024.103482

DO - 10.1016/j.xpro.2024.103482

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C2 - 39666460

AN - SCOPUS:85211490943

SN - 2666-1667

VL - 5

JO - Star Protocols

JF - Star Protocols

IS - 4

M1 - 103482

ER -

Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining

Abstract

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