Purification and analysis of growth-regulating xyloglucan-derived oligosaccharides by high-pressure liquid chromatography

Gordon J. McDougall, Stephen C. Fry*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The retention times of 10 oligosaccharides, generated from the xyloglucans of Rosa and Tropaeolum by the action of Trichoderma cellulase, and of 17 related carbohydrates, in h.p.l.c. on an amino-substituted silica (Amino-Spheri-5) depended largely on the number of hydroxyl groups per molecule, whereas h.p.l.c. on a pellicular anion-exchange resin (CarboPac PA1) was strongly influenced by the nature of the sugar residues present, especially l-fucose, and by their linkages. The major nonasaccharide (XG9, d-Glc4-d-Xyl3-d-Gal-l-Fuc) obtained from Rosa xyloglucan, after purification on Amino-Spheri-5, retained biological activity as an inhibitor of auxin-induced growth in a Pisum stem-segment bioassay. H.p.l.c. on Amino-Spheri-5 was used to monitor the action of "Driselase" in stripping the non-reducing terminal α-d-Xylp-(1→6)-β-d-Glcp units from XG9 to yield a pentasaccharide (XG5, d-Glc2-d-Xyl-d-Gal-l-Fuc).

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalCarbohydrate Research
Volume219
Issue numberC
DOIs
Publication statusPublished - 14 Oct 1991

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