Purification and culture of human blood vessel-associated progenitor cells

Mihaela Crisan, Johnny Huard, Bo Zheng, Bin Sun, Solomon Yap, Alison Logar, Jean-Paul Giacobino, Louis Casteilla, Bruno Péault

Research output: Contribution to journalLiterature reviewpeer-review

Abstract / Description of output

Multilineage progenitor cells, diversely designated as MSC, MAPC, or MDSC, have been previously extracted from long-term cultures of fetal and adult organs (e.g., bone marrow, brain, lung, pancreas, muscle, adipose tissue, and several others). The identity and location, within native tissues, of these elusive stem cells are described here. Subsets of endothelial cells and pericytes, which participate in the architecture of human blood vessels, exhibit, following purification to homogeneity, developmental multipotency. The selection from human tissues, by flow cytometry using combinations of positive and negative cell surface markers, of endothelial and perivascular cells is described here. In addition, a rare subset of myoendothelial cells that express markers of both endothelial and myogenic cell lineages and exhibit dramatic myogenic and cardiomyogenic potential has been identified and purified from skeletal muscle. The culture conditions amenable to the long-term proliferation of these blood vessel-associated stem cells in vitro are also described.

Original languageEnglish
Pages (from-to)Unit 2B.2.1-2B.2.13
JournalCurrent protocols in stem cell biology
VolumeChapter 2
DOIs
Publication statusPublished - Mar 2008

Keywords / Materials (for Non-textual outputs)

  • Adult
  • Biomarkers
  • Blood Vessels
  • Cell Culture Techniques
  • Cell Separation
  • Cells, Cultured
  • Endothelial Cells
  • Fetus
  • Flow Cytometry
  • Humans
  • Muscle, Skeletal
  • Pericytes
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells
  • Time Factors

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