TY - JOUR
T1 - Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase
AU - Seetharamappa, Jaldappagari
AU - Oke, Muse
AU - Liu, Huanting
AU - McMahon, Stephen A
AU - Johnson, Kenneth A
AU - Carter, Lester
AU - Dorward, Mark
AU - Zawadzki, Michal
AU - Overton, Ian M
AU - van Niekirk, C A Johannes
AU - Graham, Shirley
AU - Botting, Catherine H
AU - Taylor, Garry L
AU - White, Malcolm F
AU - Barton, Geoffrey J
AU - Coote, Peter J
AU - Naismith, James H
PY - 2007/6/1
Y1 - 2007/6/1
N2 - Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete data set has been collected to 2.3 A resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.
AB - Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete data set has been collected to 2.3 A resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.
KW - Bacterial Proteins
KW - Crystallization
KW - Crystallography, X-Ray
KW - Methicillin Resistance
KW - Peptide Synthases
KW - Staphylococcus aureus
U2 - 10.1107/S1744309107020362
DO - 10.1107/S1744309107020362
M3 - Article
C2 - 17554169
VL - 63
SP - 488
EP - 491
JO - Acta Crystallographica Section F Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F Structural Biology and Crystallization Communications
SN - 1744-3091
IS - Pt 6
ER -