@inbook{723a967b1d24499eaedcd686e16c8219,
title = "Purification of recombinant ESCRT-III proteins and their use in atomic force microscopy and In vitro binding and phosphorylation assays",
abstract = "The endosomal sorting complex required for transport (ESCRT)-III proteins are known to assemble into filaments that mediate membrane remodeling and fission in various biological processes, including the formation of endosomal multivesicular bodies, viral budding, cytokinesis, plasma membrane repair, nuclear pore quality control, nuclear envelope reformation, and neuron pruning. The study of the regulation and function of ESCRT-III proteins is therefore crucial to understand these events and requires a combination of in vivo and in vitro experimental techniques. Here we describe two protocols for the purification of human and Drosophila ESCRT-III proteins from bacteria and their use in in vitro phosphorylation assays and atomic force microscopy experiments on membrane lipid bilayers. These protocols can also be applied for the purification of other proteins that are insoluble when expressed in bacteria.",
keywords = "atomic force microscopy, CHMP4C, kinase assay, protein binding, protein purification",
author = "Luisa Capalbo and Ioanna Mela and Abad, {Maria Alba} and Jeyaprakash, {A. Arockia} and Edwardson, {J. Michael} and D{\textquoteright}Avino, {Pier Paolo}",
year = "2019",
month = jun,
day = "28",
doi = "10.1007/978-1-4939-9492-2_15",
language = "English",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS INC",
pages = "203--217",
booktitle = "Methods in Molecular Biology",
}