Purified Smc5/6 complex exhibits DNA substrate recognition and compaction

Pilar Gutierrez-Escribano, Silvia Hormeño, Julene Madariaga-Marcos, Roger Solé-Soler, Francis J. O’Reilly, Kyle Morris, Clara Aicart-Ramos, Ricardo Aramayo, Alex Montoya, Holger Kramer, Juri Rappsilber, Jordi Torres-Rosell, Fernando Moreno-Herrero, Luis Aragon

Research output: Contribution to journalArticlepeer-review

Abstract

Eukaryotic SMC complexes, cohesin, condensin, and Smc5/6, use ATP hydrolysis to power a plethora of functions requiring organization and restructuring of eukaryotic chromosomes in interphase and during mitosis. The Smc5/6 mechanism of action and its activity on DNA are largely unknown. Here we purified the budding yeast Smc5/6 holocomplex and characterized its core biochemical and biophysical activities. Purified Smc5/6 exhibits DNA-dependent ATP hydrolysis and SUMO E3 ligase activity. We show that Smc5/6 binds DNA topologically with affinity for supercoiled and catenated DNA templates. Employing single-molecule assays to analyze the functional and dynamic characteristics of Smc5/6 bound to DNA, we show that Smc5/6 locks DNA plectonemes and can compact DNA in an ATP-dependent manner. These results demonstrate that the Smc5/6 complex recognizes DNA tertiary structures involving juxtaposed helices and might modulate DNA topology by plectoneme stabilization and local compaction.
Original languageEnglish
Pages (from-to)1039-1054.e6
Number of pages15
JournalMolecular Cell
Volume80
Issue number6
Early online date9 Dec 2020
DOIs
Publication statusPublished - 17 Dec 2020

Keywords

  • Smc5/6 holocomplex
  • Smc5/6 purification
  • electron mmicroscopy
  • magnetic tweezers
  • single-molecule
  • DNA compaction
  • plectoneme stabilisation
  • DNA substrate recognition

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