QTL analysis and genomic selection using RADseq derived markers in Sitka spruce: the potential utility of within family data

Pablo Fuentes Utrilla, Cosmika Goswami, J.E. Cottrell, Ricardo Pong-Wong, Andrew Law, S A'Hara, Steve Lee, John Woolliams

Research output: Contribution to journalArticlepeer-review

Abstract

Sitka spruce (Picea sitchensis (Bong.) Carr) is the most common commercial plantation species in Britain and a breeding programme based on traditional lines has been in operation since the early 1960s. Rotation lengths of 40-years have led breeders to adopt a process of indirect selection at younger ages based on traits well correlated with final selection, but still the generation interval is unlikely to reduce much below twenty years. Recent successful developments with genomic selection in animal breeding have led tree breeders to consider the application of this technology. In this study a RAD sequence assay was developed as a means of investigating the potential of molecular breeding in a non-model species. DNA was extracted from nearly 500 clonally replicated trees growing in a single full-sibling family at one site in Britain. The technique proved successful in identifying 132 QTLs for 5-year bud-burst and 2 QTLs for 6-year height. In addition, the accuracy of predicting phenotypes by genomic selection was strikingly high at 0.62 and 0.59 respectively. Sensitivity analysis with 200 offspring found only a slight fall in correlation values (0.54 and 0.38) although when the training population reduced to 50 offspring predictive values fell further (0.33 and 0.25). This proved an encouraging first investigation into the potential use of genomic selection in the breeding of Sitka spruce. The authors investigate how problems associated with effective population size and linkage disequilibrium can be avoided and suggest a practical way of incorporating genomic selection into a dynamic breeding programme.
Original languageEnglish
Article number33
JournalTree Genetics and Genomes
Volume13
Issue number2
Early online date4 Feb 2017
DOIs
Publication statusE-pub ahead of print - 4 Feb 2017

Keywords

  • Sitka spruce
  • genome selection
  • RADseq
  • molecular breeding
  • height
  • bud-burst

Fingerprint Dive into the research topics of 'QTL analysis and genomic selection using RADseq derived markers in Sitka spruce: the potential utility of within family data'. Together they form a unique fingerprint.

Cite this