Quantification of abscisic acid in grapevine leaf (Vitis vinifera) by isotope-dilution liquid chromatography-mass spectrometry

Francisca Vilaró, Anna Canela-Xandri, Ramon Canela, Oriol Canela-Xandri

Research output: Contribution to journalArticlepeer-review

Abstract

A specific, sensitive, precise, and accurate method for the determination of abscisic acid (ABA) in grapevine leaf tissues is described. The method employs high-performance liquid chromatography and electrospray ionization-mass spectrometry (LC-ESI-MS) in selected ion monitoring mode (SIM) to analyze ABA using a stable isotope-labeled ABA as an internal standard. Absolute recoveries ranged from 72% to 79% using methanol/water pH 5.5 (50:50 v/v) as an extraction solvent. The best efficiency was obtained when the chromatographic separation was carried out by using a porous graphitic carbon (PGC) column. The statistical evaluation of the method was satisfactory in the work range. A relative standard deviation (RDS) of
Original languageEnglish
Pages (from-to)306-12
Number of pages7
JournalAnalytical and bioanalytical chemistry
Volume386
Issue number2
DOIs
Publication statusPublished - Sep 2006

Keywords

  • Abscisic Acid
  • Chromatography, Liquid
  • Hydrogen-Ion Concentration
  • Indicator Dilution Techniques
  • Isotopes
  • Methanol
  • Plant Leaves
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Solvents
  • Spectrometry, Mass, Electrospray Ionization
  • Vitis
  • Water

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