Projects per year
Abstract / Description of output
Quantification of Mycobacterium avium subspecies paratuberculosis (MAP) during in vitro infection experiments is challenging due to limitations of currently utilised methods, such as colony counting. Here we describe quantifying MAP infection of bovine macrophages (Mφ) using confocal microscopy. Bovine monocyte derived macrophages were infected with MAP at a high or low dose and the number of intracellular bacteria calculated at 2 hours post infection using confocal microscopy. Bacteria within simultaneously infected Mφ were quantified by colony counting in order to compare confocal microscopy results with results obtained by an established method. Confocal microscopy provided a robust alternative quantification method that allowed for assessment of the infection at the individual Mφ level. This demonstrated that MAP infection was not homogeneous, and that there were higher numbers of both infected Mφ and intracellular bacteria and bacterial aggregates at the high dose compared to the low dose, potentially impacting the Mφ response to infection. Confocal microscopy can therefore provide a level of detail regarding the infection unobtainable by other quantification methods
Original language | English |
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Pages (from-to) | 105779 |
Journal | Journal of microbiological methods |
Early online date | 19 Nov 2019 |
DOIs | |
Publication status | E-pub ahead of print - 19 Nov 2019 |
Keywords / Materials (for Non-textual outputs)
- Mycobacterium avium subspecies paratuberculosis
- quantification
- confocal microscopy
- macrophage
- infection
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Dive into the research topics of 'Quantifying Mycobacterium avium subspecies paratuberculosis infection of bovine monocyte derived macrophages by confocal microscopy'. Together they form a unique fingerprint.Projects
- 1 Finished
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EASTBIO: EASTBIO East of Scotland BioScience Doctoral Training Partnership studentship - Research Training Grant BB/J01446X/1
Smith, S.
1/09/12 → 30/09/18
Project: Other (Non-Funded/Miscellaneous)