Quantifying the contribution of BoLA-DRá3 to immune responses in a cattle cross-population

R. Baxter, N. Hastings, Y. Law, E. J. Glass

Research output: Contribution to journalMeeting abstractpeer-review

Abstract / Description of output

One approach to reducing the impact that pathogens have on livestock health and welfare is to breed for enhanced disease resistance. Suitable selectable markers may be found in genes within the Major Histocompatibility Complex (MHC), in particular the highly polymorphic MHC class II DR genes. The polymorphisms reside in exon 2 of the DR á 3 gene which encodes the peptide binding cleft. As the polymorphisms can alter the conformation of the PBC, they affect the binding efficacy of the pathogen-derived peptides, and consequently the level of the ensuing immune response. However other regions of the genome are likely to account for a significant proportion of the variability seen in disease resistance. In order to assess the relative contribution of the MHC to disease resistance and immune responsiveness in cattle, we have measured a range of immune traits in a 500 F2 Charolais-Holstein cross-population. We have improved a sequence based typing (SBT) method to reliably determine bovine MHC (BoLA) class II DR á 3 exon 2 polymorphisms, and have typed all of these cattle. Although 104 alleles have been identified for BoLA, only 22 different alleles were present in this population. Preliminary evidence from 117 animals has identified significant associations between some of the immune traits and BoLA class II DR á 3 types, and further analysis is on-going. This population has also been genotyped for 139 microsatellite markers across the genome. This information will enable us to determine the relative importance of the BoLA DR á 3 genes compared to other regions of the genome in determining immune responsiveness.
Original languageUndefined/Unknown
Pages (from-to)272
Number of pages1
JournalVeterinary Immunology and Immunopathology
Volume128
Issue number1-3
DOIs
Publication statusPublished - 2009
Event 8th International Veterinary Immunology Symposium (8th IVIS) - Ouro Preto, Brazil
Duration: 15 Aug 200719 Aug 2007

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