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Salmonella enterica is an animal and zoonotic pathogen of worldwide importance. Serovars exist that differ in their host and tissue tropism. Cattle are an important reservoir of human non-typhoidal salmonellosis and contaminated bovine peripheral lymph nodes enter the food chain via ground beef. The relative ability of different serovars to survive within the bovine lymphatic system is poorly understood and constrains the development of control strategies. This problem was addressed by developing a massively-parallel whole genome sequencing method to study mixed-serovar infections in vivo. Salmonella serovars differ genetically by naturally occurring single nucleotide polymorphisms (SNPs) in certain genes. It was hypothesised that these SNPs could be used as markers to simultaneously identify serovars in mixed populations and quantify the abundance of each member in a population. The performance of the method was validated in vitro using simulated pools containing up to 11 serovars in varying proportions. It was then applied to study serovar survival in vivo in cattle challenged orally with the same 11 serovars. All the serovars successfully colonised the bovine lymphatic system, including the peripheral lymph nodes, and thus pose a similar risk of zoonosis. This method enables the fate of multiple genetically unmodified strains to be evaluated simultaneously in a single animal. It could be useful in reducing the number of animals required to study multi-strain infections and for testing the cross-protective efficacy of vaccines and treatments. It also has the potential to be applied to diverse bacterial species which possess shared but polymorphic alleles.
- Journal Article