Quantitation of transforming growth factor-beta in plasma and pulmonary epithelial lining fluid of sheep experimentally infected with maedi-visna virus

I Begara, L Luján, L McLaren, D D Collie, H R Miller, N J Watt

Research output: Contribution to journalArticlepeer-review

Abstract

A model of experimental infection with EV1, a lytic British isolate of maedi-visna virus (MVV), was developed. Ten Texel sheep were allocated to two groups and inoculated by the respiratory route with different inocula. Six of the animals received 10(7.2) TCID50 (tissue culture infective dose) of EV1 strain, while four sheep were sham-inoculated with identically prepared virus-free buffer solution. Experimental infection was followed for 8 weeks post-inoculation (PI), with development of precipitating antibodies to MVV developed in the MVV-inoculated animals during the first 4 weeks PI. Transforming growth factor-beta (TGF-beta) levels, in both bronchoalveolar lavage fluid supernatant and plasma samples, were measured. Concentrations of pulmonary epithelial lining fluid (PELF) TGF-beta were calculated. TGF-beta concentrations in PELF were approximately 165-fold higher than in plasma. No significant differences in the concentrations of plasma or PELF TGF-beta, either within or between groups, were observed.
Original languageEnglish
Pages (from-to)261-73
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume48
Issue number3-4
Publication statusPublished - Oct 1995

Keywords

  • Animals
  • Sheep
  • Visna-maedi virus
  • Biological Assay
  • Antigens, Viral
  • Antibodies, Viral
  • Pneumonia, Progressive Interstitial, of Sheep
  • Lung
  • Bronchoalveolar Lavage Fluid
  • Epithelium
  • Transforming Growth Factor beta
  • Cell Line
  • Male

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