Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue

Andrew J Symes; Marte Eilertsen; Michael Millar; Joseph Nariculam; Alex Freeman; Maria Notara; Mark R Feneley; Hitenedra R H Patel; John R W Masters; Aamir Ahmed

doi:10.1371/journal.pone.0084295

Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue

Andrew J Symes, Marte Eilertsen, Michael Millar, Joseph Nariculam, Alex Freeman, Maria Notara, Mark R Feneley, Hitenedra R H Patel, John R W Masters, Aamir Ahmed

Deanery of Clinical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Prostate carcinoma is the most common cancer in men with few, quantifiable, biomarkers. Prostate cancer biomarker discovery has been hampered due to subjective analysis of protein expression in tissue sections. An unbiased, quantitative immunohistochemical approach provided here, for the diagnosis and stratification of prostate cancer could overcome this problem. Antibodies against four proteins BTF3, HINT1, NDRG1 and ODC1 were used in a prostate tissue array (> 500 individual tissue cores from 82 patients, 41 case pairs matched with one patient in each pair had biochemical recurrence). Protein expression, quantified in an unbiased manner using an automated analysis protocol in ImageJ software, was increased in malignant vs non-malignant prostate (by 2-2.5 fold, p

Original language	English
Pages (from-to)	e84295
Journal	PLoS ONE
Volume	8
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0084295
Publication status	Published - 2013

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10.1371/journal.pone.0084295

Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue
Copyright: © 2013 Symes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0084295

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title = "Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue",

abstract = "Prostate carcinoma is the most common cancer in men with few, quantifiable, biomarkers. Prostate cancer biomarker discovery has been hampered due to subjective analysis of protein expression in tissue sections. An unbiased, quantitative immunohistochemical approach provided here, for the diagnosis and stratification of prostate cancer could overcome this problem. Antibodies against four proteins BTF3, HINT1, NDRG1 and ODC1 were used in a prostate tissue array (> 500 individual tissue cores from 82 patients, 41 case pairs matched with one patient in each pair had biochemical recurrence). Protein expression, quantified in an unbiased manner using an automated analysis protocol in ImageJ software, was increased in malignant vs non-malignant prostate (by 2-2.5 fold, p",

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AU - Symes, Andrew J

AU - Eilertsen, Marte

AU - Millar, Michael

AU - Nariculam, Joseph

AU - Freeman, Alex

AU - Notara, Maria

AU - Feneley, Mark R

AU - Patel, Hitenedra R H

AU - Masters, John R W

AU - Ahmed, Aamir

PY - 2013

Y1 - 2013

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AB - Prostate carcinoma is the most common cancer in men with few, quantifiable, biomarkers. Prostate cancer biomarker discovery has been hampered due to subjective analysis of protein expression in tissue sections. An unbiased, quantitative immunohistochemical approach provided here, for the diagnosis and stratification of prostate cancer could overcome this problem. Antibodies against four proteins BTF3, HINT1, NDRG1 and ODC1 were used in a prostate tissue array (> 500 individual tissue cores from 82 patients, 41 case pairs matched with one patient in each pair had biochemical recurrence). Protein expression, quantified in an unbiased manner using an automated analysis protocol in ImageJ software, was increased in malignant vs non-malignant prostate (by 2-2.5 fold, p

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Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue

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