Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue

Andrew J Symes, Marte Eilertsen, Michael Millar, Joseph Nariculam, Alex Freeman, Maria Notara, Mark R Feneley, Hitenedra R H Patel, John R W Masters, Aamir Ahmed

Research output: Contribution to journalArticlepeer-review

Abstract

Prostate carcinoma is the most common cancer in men with few, quantifiable, biomarkers. Prostate cancer biomarker discovery has been hampered due to subjective analysis of protein expression in tissue sections. An unbiased, quantitative immunohistochemical approach provided here, for the diagnosis and stratification of prostate cancer could overcome this problem. Antibodies against four proteins BTF3, HINT1, NDRG1 and ODC1 were used in a prostate tissue array (> 500 individual tissue cores from 82 patients, 41 case pairs matched with one patient in each pair had biochemical recurrence). Protein expression, quantified in an unbiased manner using an automated analysis protocol in ImageJ software, was increased in malignant vs non-malignant prostate (by 2-2.5 fold, p
Original languageEnglish
Pages (from-to)e84295
JournalPLoS ONE
Volume8
Issue number12
DOIs
Publication statusPublished - 2013

Fingerprint

Dive into the research topics of 'Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue'. Together they form a unique fingerprint.

Cite this