Quantitative analysis of multiple antibody-ligand interactions in a microchip using fluorescence polarization anisotropy

K. Eyer, T. Robinson, P. Kuhn, P. S. Dittrich*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a microfluidic device that allows quantitative analysis of protein interactions with fluorescently labeled ligands. Dissociation constants (Kd), binding classes (m) as well as number of binding sites (n) can be determined in the same experiment, even if protein mixtures are present.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages500-502
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords / Materials (for Non-textual outputs)

  • Binding studies
  • Dissociation constant
  • Protein-ligand interaction

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