Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

N. Z. Homer, R. M. Reynolds, C. Mattson, M. A. Bailey, B. R. Walker, Ruth Andrew

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.
Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalJournal of Chromatography B
Volume877
Issue number5-6
DOIs
Publication statusPublished - Feb 2009

Keywords

  • RU38486
  • Mifepristone
  • Steroid
  • Mass spectrometry
  • Liquid chromatography

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