Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

N. Z. Homer; R. M. Reynolds; C. Mattson; M. A. Bailey; B. R. Walker; Ruth Andrew

doi:10.1016/j.jchromb.2008.12.051

Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

N. Z. Homer, R. M. Reynolds, C. Mattson, M. A. Bailey, B. R. Walker, Ruth Andrew

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

Original language	English
Pages (from-to)	497-501
Number of pages	5
Journal	Journal of Chromatography B
Volume	877
Issue number	5-6
DOIs	https://doi.org/10.1016/j.jchromb.2008.12.051
Publication status	Published - Feb 2009

Keywords / Materials (for Non-textual outputs)

RU38486
Mifepristone
Steroid
Mass spectrometry
Liquid chromatography

Access to Document

10.1016/j.jchromb.2008.12.051

http://www.sciencedirect.com/science/article/pii/S1570023208009483

Cite this

@article{7e0190aba9ef4334b01202ac59eecce7,

title = "Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry",

abstract = "A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.",

keywords = "RU38486, Mifepristone, Steroid, Mass spectrometry, Liquid chromatography",

author = "Homer, {N. Z.} and Reynolds, {R. M.} and C. Mattson and Bailey, {M. A.} and Walker, {B. R.} and Ruth Andrew",

year = "2009",

month = feb,

doi = "10.1016/j.jchromb.2008.12.051",

language = "English",

volume = "877",

pages = "497--501",

journal = "Journal of Chromatography B",

issn = "1570-0232",

publisher = "Elsevier",

number = "5-6",

}

TY - JOUR

T1 - Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

AU - Homer, N. Z.

AU - Reynolds, R. M.

AU - Mattson, C.

AU - Bailey, M. A.

AU - Walker, B. R.

AU - Andrew, Ruth

PY - 2009/2

Y1 - 2009/2

N2 - A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

AB - A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

KW - RU38486

KW - Mifepristone

KW - Steroid

KW - Mass spectrometry

KW - Liquid chromatography

UR - http://www.scopus.com/inward/record.url?scp=59249102751&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2008.12.051

DO - 10.1016/j.jchromb.2008.12.051

M3 - Article

SN - 1570-0232

VL - 877

SP - 497

EP - 501

JO - Journal of Chromatography B

JF - Journal of Chromatography B

IS - 5-6

ER -

Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Cite this