Projects per year
Abstract / Description of output
Quantitative cross-linking/mass spectrometry (QCLMS) probes protein structural dynamics in solution by quantitatively comparing the yields of cross-links between different conformational statuses. We have used QCLMS to understand the final maturation step of the proteasome lid and also to elucidate the structure of complement C3(H2O). Here we benchmark our workflow using a structurally well-described reference system, the human complement protein C3 and its activated cleavage product C3b. We found that small local conformational changes affect the yields of cross-linking residues that are near in space while larger conformational changes affect the detectability of cross-links. Distinguishing between minor and major changes required robust analysis based on replica analysis and a label-swapping procedure. By providing workflow, code of practice and a framework for semi-automated data processing, we lay the foundation for QCLMS as a tool to monitor the domain choreography that drives binary switching in many protein-protein interaction networks.
Original language | English |
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Number of pages | 19 |
Journal | Wellcome Open Research |
DOIs | |
Publication status | Published - 15 Nov 2016 |
Keywords / Materials (for Non-textual outputs)
- cross-linking/mass spectrometry
- quantitative protein structure
- conformational change
- isotope labeled cross-linkers
- automated data process
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Dive into the research topics of 'Quantitative cross-linking/mass spectrometry reveals subtle protein conformational changes: QCLMS reveals protein conformational changes'. Together they form a unique fingerprint.Projects
- 5 Finished
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Protein structures in the context of time and space by mass spectrometry
1/06/14 → 31/05/21
Project: Research
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Core funding renewal for the Wellcome Trust Centre for Cell Biology
1/10/11 → 30/04/17
Project: Research