Quantitative immunoassays for diagnosis and carrier detection in cystic fibrosis

S Bullock, C Hayward, J Manson, D J Brock, J A Raeburn

Research output: Contribution to journalArticlepeer-review

Abstract

Quantitative immunoprecipitation and immunoradiometric assays have been developed for a protein present in the serum of cystic fibrosis homozygotes, and to a lesser extent in the serum of heterozygotes. When tested on a panel of sera from 14 cystic fibrosis patients, 29 heterozygotes and 23 controls, the immunoprecipitation assay allowed correct assignments to be made on 94% of occasions with one batch of antiserum and 95% with another. With the same panel of sera, the immunoradiometric assay allowed 94% correct assignments. It is suggested that such accuracy is the maximum that can be expected in the present state of knowledge of cystic fibrosis.

Original languageEnglish
Pages (from-to)336-41
Number of pages6
JournalClinical genetics
Volume21
Issue number5
DOIs
Publication statusPublished - May 1982

Keywords

  • Blood Proteins
  • Cystic Fibrosis
  • Heterozygote Detection
  • Homozygote
  • Humans
  • Immunoelectrophoresis
  • Radioimmunoassay
  • Reference Values

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