Quantitative proteomic analysis of the exoelectrogenic bacterium Arcobacter butzleri ED-1 reveals increased abundance of a flagellin protein under anaerobic growth on an insoluble electrode

Ana G. Pereira-Medrano, Matthew C. Knighton, Gregory J.S. Fowler, Zi Yen Ler, Trong Khoa Pham, Saw Yen Ow, Andrew Free, Bruce Ward, Phillip C. Wright

Research output: Contribution to journalArticlepeer-review

Abstract

Exoelectrogens have the ability to generate electricity in mediator-less microbial fuel cells (MFCs) by extracellular electron transfer to the anode. We investigate the anode-specific responses of Arcobacter butzleri ED-1, the first identified exoelectrogenic Epsilonproteobacterium. iTRAQ and 2D-LC MS/MS driven proteomics were used to compare protein abundances in A. butzleri ED-1 when generating an electronegative potential (−225 mV) in an anaerobic
half-cell—either growing as an electrogenic biofilm or suspended in the liquidmedium - versus a microaerobic culture. This is the first quantitative proteomic study concentrating on growth of an exoelectrogen during current generation. From 720 proteins identified and quantified (soluble and insoluble sub-proteomes), statistical analysis reveals 75 differentially-expressed
proteins. This dataset was enriched in proteins regulating energy and intermediary metabolism, electron and protein transport. Flagellin up-regulation was concomitant with electron transport in the anodic cells, while decreased abundance of a methyl-accepting chemotaxis protein
suggested that flagella were involved in communication with the anode surface and electrogenesis, rather than motility. Two novel cytochromes potentially related to electron transportwere up-regulated in anaerobic cultures.We demonstrate that employing an insoluble extracellular electron acceptor for anaerobic growth regulatesmultiple proteins involved in cell surface properties, electron transport and the methylcitrate cycle.
Original languageEnglish
Pages (from-to)197-210
Number of pages14
JournalJournal of proteomics
Volume78
DOIs
Publication statusPublished - Jan 2013

Keywords

  • Arcobacter butzleri ED-1
  • Proteome
  • iTRAQ
  • Exoelectrogenesis
  • Microbial fuel cell
  • Biofilm

Fingerprint

Dive into the research topics of 'Quantitative proteomic analysis of the exoelectrogenic bacterium Arcobacter butzleri ED-1 reveals increased abundance of a flagellin protein under anaerobic growth on an insoluble electrode'. Together they form a unique fingerprint.

Cite this