R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes

Konstantina Skourti-Stathaki; Elena Torlai Triglia; Marie Warburton; Philipp Voigt; Adrian Bird; Ana Pombo

doi:10.1016/j.molcel.2018.12.016

R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes

Konstantina Skourti-Stathaki^*, Elena Torlai Triglia, Marie Warburton, Philipp Voigt, Adrian Bird, Ana Pombo

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Skourti-Stathaki and colleagues demonstrate that a subset of Polycomb-repressed genes form R-loops genome-wide. At these genes, R-loop loss leads to reduced Polycomb (PRC1 and PRC2) recruitment, enhanced polymerase II activation, and transcriptional derepression. Derepression increases in conditions of reduced PRC1 recruitment, suggesting that R-loops and PRC1 both contribute to Polycomb repression.

Original language	English
Pages (from-to)	930-945
Number of pages	21
Journal	Molecular Cell
Volume	73
Issue number	5
DOIs	https://doi.org/10.1016/j.molcel.2018.12.016
Publication status	Published - 7 Mar 2019

Keywords / Materials (for Non-textual outputs)

gene regulation
poising
Polycomb proteins
R loops
RING1B
RNA polymerase II

Access to Document

10.1016/j.molcel.2018.12.016

R-loops enhance AAM Skourti-Stathaki_Main text Mol. Cell_final KSSAccepted author manuscript, 334 KBLicence: CC BY-NC-ND
R-Loops Enhance Polycomb PUB OA CC BY 1-s2.0-S1097276518310700-main
© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 6.48 MBLicence: CC BY

Cite this

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title = "R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes",

abstract = "Skourti-Stathaki and colleagues demonstrate that a subset of Polycomb-repressed genes form R-loops genome-wide. At these genes, R-loop loss leads to reduced Polycomb (PRC1 and PRC2) recruitment, enhanced polymerase II activation, and transcriptional derepression. Derepression increases in conditions of reduced PRC1 recruitment, suggesting that R-loops and PRC1 both contribute to Polycomb repression.",

keywords = "gene regulation, poising, Polycomb proteins, R loops, RING1B, RNA polymerase II",

author = "Konstantina Skourti-Stathaki and {Torlai Triglia}, Elena and Marie Warburton and Philipp Voigt and Adrian Bird and Ana Pombo",

year = "2019",

month = mar,

day = "7",

doi = "10.1016/j.molcel.2018.12.016",

language = "English",

volume = "73",

pages = "930--945",

journal = "Molecular Cell",

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T1 - R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes

AU - Skourti-Stathaki, Konstantina

AU - Torlai Triglia, Elena

AU - Warburton, Marie

AU - Voigt, Philipp

AU - Bird, Adrian

AU - Pombo, Ana

PY - 2019/3/7

Y1 - 2019/3/7

N2 - Skourti-Stathaki and colleagues demonstrate that a subset of Polycomb-repressed genes form R-loops genome-wide. At these genes, R-loop loss leads to reduced Polycomb (PRC1 and PRC2) recruitment, enhanced polymerase II activation, and transcriptional derepression. Derepression increases in conditions of reduced PRC1 recruitment, suggesting that R-loops and PRC1 both contribute to Polycomb repression.

AB - Skourti-Stathaki and colleagues demonstrate that a subset of Polycomb-repressed genes form R-loops genome-wide. At these genes, R-loop loss leads to reduced Polycomb (PRC1 and PRC2) recruitment, enhanced polymerase II activation, and transcriptional derepression. Derepression increases in conditions of reduced PRC1 recruitment, suggesting that R-loops and PRC1 both contribute to Polycomb repression.

KW - gene regulation

KW - poising

KW - Polycomb proteins

KW - R loops

KW - RING1B

KW - RNA polymerase II

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U2 - 10.1016/j.molcel.2018.12.016

DO - 10.1016/j.molcel.2018.12.016

M3 - Article

C2 - 30709709

AN - SCOPUS:85062374840

SN - 1097-2765

VL - 73

SP - 930

EP - 945

JO - Molecular Cell

JF - Molecular Cell

IS - 5

ER -

R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Wellcome Centre for Cell Biology

Towards understanding and treatment of MeCP2-related disorders

PRCTOERC: Novel Regulatory Principles of Polycomb Repressive Complex 2

R-loops and PRC1 repress Polycomb-target genes in mouse embryonic stem cells

Cite this

R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

Wellcome Centre for Cell Biology

Towards understanding and treatment of MeCP2-related disorders

PRCTOERC: Novel Regulatory Principles of Polycomb Repressive Complex 2

Datasets

R-loops and PRC1 repress Polycomb-target genes in mouse embryonic stem cells

Cite this