Rac1 drives intestinal stem cell proliferation and regeneration

Kevin B Myant, Alessandro Scopelliti, Sara Haque, Marcos Vidal, Owen J Sansom, Julia B Cordero

Research output: Contribution to journalArticlepeer-review

Abstract

Adult stem cells are responsible for maintaining the balance between cell proliferation and differentiation within self-renewing tissues. The molecular and cellular mechanisms mediating such balance are poorly understood. The production of reactive oxygen species (ROS) has emerged as an important mediator of stem cell homeostasis in various systems. Our recent work demonstrates that Rac1-dependent ROS production mediates intestinal stem cell (ISC) proliferation in mouse models of colorectal cancer (CRC). Here, we use the adult Drosophila midgut and the mouse small intestine to directly address the role of Rac1 in ISC proliferation and tissue regeneration in response to damage. Our results demonstrate that Rac1 is necessary and sufficient to drive ISC proliferation and regeneration in an ROS-dependent manner. Our data point to an evolutionarily conserved role of Rac1 in intestinal homeostasis and highlight the value of combining work in the mammalian and Drosophila intestine as paradigms to study stem cell biology.

Original languageEnglish
Pages (from-to)2973-7
Number of pages5
JournalCell Cycle
Volume12
Issue number18
DOIs
Publication statusPublished - 15 Sep 2013

Keywords

  • Animals
  • Cell Proliferation
  • Drosophila
  • Drosophila Proteins
  • Hydrogen-Ion Concentration
  • Intestines
  • Mice
  • Mice, Inbred C57BL
  • Oxidative Stress
  • RNA Interference
  • Reactive Oxygen Species
  • Regeneration
  • Stem Cells
  • rac1 GTP-Binding Protein

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