Rapid and specific biotin labelling of the erythrocyte surface antigens of both cultured and ex-vivo Plasmodium parasites

Lisa Sharling; Kordai Mp Sowa; Joanne Thompson; Helen M Kyriacou; David E Arnot

doi:10.1186/1475-2875-6-66

Rapid and specific biotin labelling of the erythrocyte surface antigens of both cultured and ex-vivo Plasmodium parasites

Lisa Sharling, Kordai Mp Sowa, Joanne Thompson, Helen M Kyriacou, David E Arnot

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Sensitive detection of parasite surface antigens expressed on erythrocyte membranes is necessary to further analyse the molecular pathology of malaria. This study describes a modified biotin labelling/osmotic lysis method which rapidly produces membrane extracts enriched for labelled surface antigens and also improves the efficiency of antigen recovery compared with traditional detergent extraction and surface radio-iodination. The method can also be used with ex-vivo parasites.

Original language	English
Number of pages	7
Journal	Malaria Journal
Volume	6
Issue number	66
DOIs	https://doi.org/10.1186/1475-2875-6-66
Publication status	Published - 2007

Access to Document

10.1186/1475-2875-6-66

Open Access PDF available here.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.05 MB

EUROMALVAC2
Arnot, D.
EU government bodies
1/09/02 → 31/05/06
Project: Research

Cite this

@article{a5b3f44263c54b3e976001997798a4b4,

title = "Rapid and specific biotin labelling of the erythrocyte surface antigens of both cultured and ex-vivo Plasmodium parasites",

abstract = "Sensitive detection of parasite surface antigens expressed on erythrocyte membranes is necessary to further analyse the molecular pathology of malaria. This study describes a modified biotin labelling/osmotic lysis method which rapidly produces membrane extracts enriched for labelled surface antigens and also improves the efficiency of antigen recovery compared with traditional detergent extraction and surface radio-iodination. The method can also be used with ex-vivo parasites.",

author = "Lisa Sharling and Sowa, {Kordai Mp} and Joanne Thompson and Kyriacou, {Helen M} and Arnot, {David E}",

year = "2007",

doi = "10.1186/1475-2875-6-66",

language = "English",

volume = "6",

journal = "Malaria Journal",

issn = "1475-2875",

publisher = "BMC",

number = "66",

}

TY - JOUR

T1 - Rapid and specific biotin labelling of the erythrocyte surface antigens of both cultured and ex-vivo Plasmodium parasites

AU - Sharling, Lisa

AU - Sowa, Kordai Mp

AU - Thompson, Joanne

AU - Kyriacou, Helen M

AU - Arnot, David E

PY - 2007

Y1 - 2007

N2 - Sensitive detection of parasite surface antigens expressed on erythrocyte membranes is necessary to further analyse the molecular pathology of malaria. This study describes a modified biotin labelling/osmotic lysis method which rapidly produces membrane extracts enriched for labelled surface antigens and also improves the efficiency of antigen recovery compared with traditional detergent extraction and surface radio-iodination. The method can also be used with ex-vivo parasites.

AB - Sensitive detection of parasite surface antigens expressed on erythrocyte membranes is necessary to further analyse the molecular pathology of malaria. This study describes a modified biotin labelling/osmotic lysis method which rapidly produces membrane extracts enriched for labelled surface antigens and also improves the efficiency of antigen recovery compared with traditional detergent extraction and surface radio-iodination. The method can also be used with ex-vivo parasites.

U2 - 10.1186/1475-2875-6-66

DO - 10.1186/1475-2875-6-66

M3 - Article

C2 - 17519013

SN - 1475-2875

VL - 6

JO - Malaria Journal

JF - Malaria Journal

IS - 66

ER -

Rapid and specific biotin labelling of the erythrocyte surface antigens of both cultured and ex-vivo Plasmodium parasites

Abstract / Description of output

Access to Document

Fingerprint

Projects

EUROMALVAC2

Cite this