Rapid detection and differentiation of Erysipelothrix spp. by a novel multiplex real-time PCR assay

N. Pal, J.S. Bender, T. Opriessnig

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: The aim of this study was to develop a multiplex real-time PCR assay for the identification and discrimination of Erysipelothrix rhusiopathiae, tonsillarum and Erysipelothrix sp. strain 2 for direct detection of Erysipelothrix spp. from animal specimens. Methods and Results: A primer set and three species-specific probes with different end labelling were designed from the noncoding region downstream of the 5S rRNA coding region. The sensitivity, specificity and repeatability of the assay were validated by analysing 27 Erysipelothrix spp. reference serotype strains and ten septicemia-associated non-Erysipelothrix spp. bacterial isolates. Cross-reactivity with Erysipelothrix sp. strain 1 was not observed with any of the primer probe combinations. The detection limit was determined to be
Original languageEnglish
Pages (from-to)1083-1093
Number of pages11
JournalJournal of Applied Microbiology
Volume108
Issue number3
DOIs
Publication statusPublished - 1 Mar 2010

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