Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe

Richa Sharma; Harinee Rajagopalan; Maxime Klausen; Mani Vimalin Jeyalatha; Muhammed Üçüncü; Seshasailam Venkateswaran; Appakkudal R. Anand; Mark Bradley

doi:10.1039/D2SD00061J

Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe

Richa Sharma, Harinee Rajagopalan, Maxime Klausen, Mani Vimalin Jeyalatha, Muhammed Üçüncü, Seshasailam Venkateswaran, Appakkudal R. Anand, Mark Bradley

Research output: Contribution to journal › Article › peer-review

Abstract

Bacterial infections of the eye are significant causes of morbidity that can lead to permanent visual loss without rapid and adequate treatment, with Gram-positive bacteria causing the majority of ocular infections. Here we report a novel probe, based on the reductive amination of vancomycin with a 4-nitrobenzoxadiazole (NBD)-based aldehyde, that rapidly and specifically detects Gram positive infections from ocular samples.

Original language	English
Pages (from-to)	1014-1020
Journal	Sensors & Diagnostics
Volume	1
Issue number	5
Early online date	30 Jun 2022
DOIs	https://doi.org/10.1039/D2SD00061J
Publication status	E-pub ahead of print - 30 Jun 2022

Access to Document

10.1039/D2SD00061J

20220630Bradleyd2sd00061jVoRFinal published version, 1.84 MBLicence: Creative Commons: Attribution (CC-BY)

http://xlink.rsc.org/?DOI=D2SD00061J

Detect and Destroy - Theranostic Smart Probes for Fungal Infections
Bradley, M. (Principal Investigator)
Other (Learned Society)
1/11/20 → 31/10/22
Project: Research
EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond
Bradley, M. (Principal Investigator), Bradley, M. (Principal Investigator), Dhaliwal, K. (Co-investigator), Dhaliwal, K. (Co-investigator), Haslett, C. (Co-investigator), Henderson, R. (Co-investigator), Walsh, T. (Co-investigator) & Walsh, T. (Co-investigator)
Engineering and Physical Sciences Research Council
1/01/19 → 1/06/23
Project: Research
Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
Bradley, M. (Principal Investigator)
EPSRC
1/10/13 → 31/03/19
Project: Research

Cite this

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title = "Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe",

abstract = "Bacterial infections of the eye are significant causes of morbidity that can lead to permanent visual loss without rapid and adequate treatment, with Gram-positive bacteria causing the majority of ocular infections. Here we report a novel probe, based on the reductive amination of vancomycin with a 4-nitrobenzoxadiazole (NBD)-based aldehyde, that rapidly and specifically detects Gram positive infections from ocular samples.",

author = "Richa Sharma and Harinee Rajagopalan and Maxime Klausen and Jeyalatha, \{Mani Vimalin\} and Muhammed {\"U}{\c c}{\"u}nc{\"u} and Seshasailam Venkateswaran and Anand, \{Appakkudal R.\} and Mark Bradley",

year = "2022",

month = jun,

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doi = "10.1039/D2SD00061J",

language = "English",

volume = "1",

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journal = "Sensors \& Diagnostics",

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AU - Sharma, Richa

AU - Rajagopalan, Harinee

AU - Klausen, Maxime

AU - Jeyalatha, Mani Vimalin

AU - Üçüncü, Muhammed

AU - Venkateswaran, Seshasailam

AU - Anand, Appakkudal R.

AU - Bradley, Mark

PY - 2022/6/30

Y1 - 2022/6/30

N2 - Bacterial infections of the eye are significant causes of morbidity that can lead to permanent visual loss without rapid and adequate treatment, with Gram-positive bacteria causing the majority of ocular infections. Here we report a novel probe, based on the reductive amination of vancomycin with a 4-nitrobenzoxadiazole (NBD)-based aldehyde, that rapidly and specifically detects Gram positive infections from ocular samples.

AB - Bacterial infections of the eye are significant causes of morbidity that can lead to permanent visual loss without rapid and adequate treatment, with Gram-positive bacteria causing the majority of ocular infections. Here we report a novel probe, based on the reductive amination of vancomycin with a 4-nitrobenzoxadiazole (NBD)-based aldehyde, that rapidly and specifically detects Gram positive infections from ocular samples.

U2 - 10.1039/D2SD00061J

DO - 10.1039/D2SD00061J

M3 - Article

SN - 2635-0998

VL - 1

SP - 1014

EP - 1020

JO - Sensors & Diagnostics

JF - Sensors & Diagnostics

IS - 5

ER -

Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe

Abstract

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Projects

Detect and Destroy - Theranostic Smart Probes for Fungal Infections

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond

Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging

Cite this