Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

Dawn Gillies, Wesam Gamal, Andrew Downes, Yvonne Reinwald, Ying Yang, Alicia El Haj, Pierre Bagnaninchi

Research output: Contribution to journalArticlepeer-review

Abstract

Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed.

We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D.

We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.
Original languageEnglish
Number of pages8
JournalMethods
Volume136
Early online date31 Oct 2017
DOIs
Publication statusPublished - Mar 2018

Keywords

  • Optical coherence phase microscopy
  • Mechanical behaviour
  • Real-time monitoring
  • Hydrostatic pressure
  • Phase imaging

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