Projects per year
Abstract
Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed.
We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D.
We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.
We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D.
We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.
Original language | English |
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Number of pages | 8 |
Journal | Methods |
Volume | 136 |
Early online date | 31 Oct 2017 |
DOIs | |
Publication status | Published - Mar 2018 |
Keywords / Materials (for Non-textual outputs)
- Optical coherence phase microscopy
- Mechanical behaviour
- Real-time monitoring
- Hydrostatic pressure
- Phase imaging
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Dive into the research topics of 'Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy'. Together they form a unique fingerprint.Projects
- 1 Finished
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A HUB FOR ENGINEERING AND EXPLOITING THE STEM CELL NICHE
Forbes, S. (Principal Investigator)
1/11/13 → 31/03/18
Project: Research