Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Jamie Marland, Mark Gray, Camelia Dunare, Ewen Blair, Andreas Tsiamis, Paul Sullivan, Eva González-Fernández, Stephen Greenhalgh, Rachael Gregson, Eddie Clutton, Magdalena Parys, Alex Dyson, Mervyn Singer, Ian Kunkler, Mark A Potter, Srinjoy Mitra, Jonathan Terry, Stewart Smith, Andrew Mount, Ian UnderwoodAnthony Walton, David Argyle, Alan Murray

Research output: Contribution to journalArticlepeer-review

Abstract

Hypoxia commonly occurs within tumours and is a major cause of radiotherapy resistance. Clinical outcomes could be improved by locating and selectively increasing the dose delivered to hypoxic regions. Here we describe a miniature implantable sensor for real-time monitoring of tissue oxygenation that could enable this novel treatment approach to be implemented. The sensor uses a solid-state electrochemical cell that was microfabricated at wafer level on a silicon substrate, and includes an integrated reference electrode and electrolyte membrane. It gave a linear response to oxygen concentration, and was unaffected by sterilisation and irradiation, but showed susceptibility to biofouling. Oxygen selectivity was also evaluated against various clinically relevant electroactive compounds. We investigated its robustness and functionality under realistic clinical conditions using a sheep model of lung cancer. The sensor remained functional following CT-guided tumour implantation, and was sufficiently sensitive to track acute changes in oxygenation within tumour tissue.
Original languageEnglish
Article number100375
Pages (from-to)1-12
Number of pages12
JournalSensing and Bio-Sensing Research
Volume30
Early online date11 Aug 2020
DOIs
Publication statusE-pub ahead of print - 11 Aug 2020

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