Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

John Chu, Marcos Gonzalez-Lopez, Scott L. Cockroft, Manuel Amorin, M. Reza Ghadiri

Research output: Contribution to journalArticlepeer-review

Abstract

A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture).
Original languageEnglish
Pages (from-to)10106-10109
JournalAngewandte Chemie International Edition
Volume49
Issue number52
DOIs
Publication statusPublished - Dec 2010

Keywords

  • DNA sequencing
  • α-hemolysin
  • nanopores
  • single-molecule studies
  • supramolecular chemistry

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