Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

John Chu; Marcos Gonzalez-Lopez; Scott L. Cockroft; Manuel Amorin; M. Reza Ghadiri

doi:10.1002/anie.201005460

Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

John Chu, Marcos Gonzalez-Lopez, Scott L. Cockroft, Manuel Amorin, M. Reza Ghadiri

Research output: Contribution to journal › Article › peer-review

Abstract

A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture).

Original language	English
Pages (from-to)	10106-10109
Journal	Angewandte Chemie International Edition
Volume	49
Issue number	52
DOIs	https://doi.org/10.1002/anie.201005460
Publication status	Published - Dec 2010

Keywords / Materials (for Non-textual outputs)

DNA sequencing
α-hemolysin
nanopores
single-molecule studies
supramolecular chemistry

Access to Document

10.1002/anie.201005460

PDF available hereAccepted author manuscript, 1.17 MB

http://onlinelibrary.wiley.com/doi/10.1002/anie.201005460/abstract

Cite this

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title = "Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore",

abstract = "A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture).",

keywords = "DNA sequencing , α-hemolysin , nanopores , single-molecule studies , supramolecular chemistry ",

author = "John Chu and Marcos Gonzalez-Lopez and Cockroft, \{Scott L.\} and Manuel Amorin and Ghadiri, \{M. Reza\}",

year = "2010",

month = dec,

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language = "English",

volume = "49",

pages = "10106--10109",

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AU - Chu, John

AU - Gonzalez-Lopez, Marcos

AU - Cockroft, Scott L.

AU - Amorin, Manuel

AU - Ghadiri, M. Reza

PY - 2010/12

Y1 - 2010/12

N2 - A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture).

AB - A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture).

KW - DNA sequencing

KW - α-hemolysin

KW - nanopores

KW - single-molecule studies

KW - supramolecular chemistry

UR - https://www.scopus.com/pages/publications/78650483427

U2 - 10.1002/anie.201005460

DO - 10.1002/anie.201005460

M3 - Article

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JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 52

ER -

Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

Abstract

Keywords / Materials (for Non-textual outputs)

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Nanopore-based single-molecule biophysics: Probing enzyme conformational dynamics

Invited Lecture at European-Winter School on Physical Organic Chemistry

Cite this

Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

Nanopore-based single-molecule biophysics: Probing enzyme conformational dynamics

Activities

Invited Lecture at European-Winter School on Physical Organic Chemistry

Cite this