RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica

Albert Mayola; Oihane Irazoki; Ignacio A Martínez; Dmitri Petrov; Filippo Menolascina; Roman Stocker; José A Reyes-Darias; Tino Krell; Jordi Barbé; Susana Campoy

doi:10.1371/journal.pone.0105578

RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica

Albert Mayola, Oihane Irazoki, Ignacio A Martínez, Dmitri Petrov, Filippo Menolascina, Roman Stocker, José A Reyes-Darias, Tino Krell, Jordi Barbé, Susana Campoy

School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium.

Original language	English
Pages (from-to)	e105578
Journal	PLoS ONE
Volume	9
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0105578
Publication status	Published - 2014

Keywords / Materials (for Non-textual outputs)

Bacterial Proteins
Chemotaxis
Flagella
Gene Deletion
Mutation
Protein Binding
Rec A Recombinases
Salmonella enterica
Salmonella typhimurium

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10.1371/journal.pone.0105578

Filippo Menolascina
- School of Engineering - Personal Chair of Engineering Biology
- Centre for Engineering Biology
- Bayes Centre - Director of Research
Person: Academic: Research Active

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title = "RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica",

abstract = "The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium.",

keywords = "Bacterial Proteins, Chemotaxis, Flagella, Gene Deletion, Mutation, Protein Binding, Rec A Recombinases, Salmonella enterica, Salmonella typhimurium",

author = "Albert Mayola and Oihane Irazoki and Mart{\'i}nez, {Ignacio A} and Dmitri Petrov and Filippo Menolascina and Roman Stocker and Reyes-Darias, {Jos{\'e} A} and Tino Krell and Jordi Barb{\'e} and Susana Campoy",

year = "2014",

doi = "10.1371/journal.pone.0105578",

language = "English",

volume = "9",

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TY - JOUR

T1 - RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica

AU - Mayola, Albert

AU - Irazoki, Oihane

AU - Martínez, Ignacio A

AU - Petrov, Dmitri

AU - Menolascina, Filippo

AU - Stocker, Roman

AU - Reyes-Darias, José A

AU - Krell, Tino

AU - Barbé, Jordi

AU - Campoy, Susana

PY - 2014

Y1 - 2014

N2 - The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium.

AB - The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium.

KW - Bacterial Proteins

KW - Chemotaxis

KW - Flagella

KW - Gene Deletion

KW - Mutation

KW - Protein Binding

KW - Rec A Recombinases

KW - Salmonella enterica

KW - Salmonella typhimurium

U2 - 10.1371/journal.pone.0105578

DO - 10.1371/journal.pone.0105578

M3 - Article

C2 - 25147953

SN - 1932-6203

VL - 9

SP - e105578

JO - PLoS ONE

JF - PLoS ONE

IS - 8

ER -

RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Filippo Menolascina

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