Projects per year
Abstract / Description of output
DNA double-strand break (DSB) repair is critical for cell survival. A diverse range of organisms from bacteria to humans rely on homologous recombination for accurate DSB repair. This requires both coordinate action of the two ends of a DSB and stringent control of the resultant DNA replication to prevent unwarranted DNA amplification and aneuploidy. In Escherichia coli, RecBCD enzyme is responsible for the initial steps of homologous recombination. Previous work has revealed recD mutants to be nuclease defective but recombination proficient. Despite this proficiency, we show here that a recD null mutant is defective for the repair of a two-ended DSB and that this defect is associated with unregulated chromosome amplification and defective chromosome segregation. Our results demonstrate that RecBCD plays an important role in avoiding this amplification by coordinating the two recombining ends in a manner that prevents divergent replication forks progressing away from the DSB site.
Original language | English |
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Pages (from-to) | 6670-6682 |
Number of pages | 13 |
Journal | Nucleic Acids Research |
Volume | 46 |
Issue number | 13 |
Early online date | 13 Jun 2018 |
DOIs | |
Publication status | Published - 27 Jul 2018 |
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Dive into the research topics of 'RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplification'. Together they form a unique fingerprint.Projects
- 5 Finished
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DNA Misfolding and the Maintenance of Genome Stability: an Integrated Molecular, Cellular and Genomic Investigation of DNA Double-Strand Break Repair
Leach, D.
1/07/15 → 31/12/20
Project: Research
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Future-Proofing the sustainability of the MRC high throughput sequencing hub in Scotland
Blaxter, M.
1/10/12 → 30/09/14
Project: Research
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