Reconstitution of B cell subsets in Rag deficient mice by transplantation of in vitro differentiated embryonic stem cells

A J Potocnik, G Nerz, H Kohler, K Eichmann

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro differentiated embryonic stem (ES) cells contain a population which is similar to fetal liver pro/pre-B cells on the basis of cell surface antigens and cytoplasmic expression of immunoglobin heavy chain. This population was purified and transplanted into Rag-1 deficient recipients to characterize its developmental potential in vivo. Following intravenous transfer, these cells rapidly reconstituted the splenic B but not the T cell compartment. Reconstitution was transient, indicating the lack of long-term reconstituting capacity. Similar to fetal liver, B-1 type as well as conventional B cells were generated, accompanied by high serum IgM levels. Intraperitoneal injection generated high numbers of peritoneal B cells, predominately of the B-1a phenotype, with poor splenic repopulation and low serum IgM levels. These observations suggest the emergence of two different B lineage precursor populations during in vitro ES cell differentiation and define a possible role of the microenvironment in directing lymphoid development.

Original languageEnglish
Pages (from-to)131-7
Number of pages7
JournalImmunology Letters
Volume57
Issue number1-3
Publication statusPublished - 1 Jun 1997

Keywords

  • Animals
  • Antigens, CD45
  • Antigens, CD5
  • B-Lymphocyte Subsets
  • Cell Differentiation
  • Cell Line
  • DNA-Binding Proteins
  • Female
  • Genes, RAG-1
  • Hematopoietic Stem Cell Transplantation
  • Homeodomain Proteins
  • Immunoglobulin D
  • Immunoglobulin M
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Stem Cells

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