Reconstitution of the pyridoxal 5'-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis

Ashley E Beattie, David J Clarke, John M Wadsworth, Jonathan Lowther, Ho-Lam Sin, Dominic J Campopiano

Research output: Contribution to journalArticlepeer-review

Abstract

The pyridoxal 5'-phosphate (PLP)-dependent enzyme serine palmitoyltransferase (SPT) is required for de novo sphingolipid biosynthesis. A previous study revealed a novel and unexpected interaction between the hydroxyl group of the l-serine substrate and the 5'-phosphate group of PLP. By using pyridoxal (PL), the dephosphorylated analogue of vitamin B6, we show here that this interaction is important for substrate specificity and optimal catalytic efficiency.
Original languageEnglish
Pages (from-to)7058-60
Number of pages3
JournalChemical Communications
Volume49
Issue number63
Early online date24 Jun 2013
DOIs
Publication statusPublished - 14 Aug 2013

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