Recovery of nucleus basalis cholinergic neurons by grafting NGF secretor fibroblasts

P Piccardo, D Maysinger, A C Cuello

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The effects of nerve growth factor (NGF) secreted from genetically modified fibroblasts was studied in vitro, using dissociated septal cells and in vivo, in rats bearing unilateral cortical devascularizing lesions. Transfected fibroblasts expressing nerve growth factor (NGF) were co-cultured with rat embryonic cholinergic cells of the septal region. This in vitro system showed that NGF secretor cells produce biologically active NGF, as determined by increasing choline acetyltransferase (ChAT) activity in septal culture after seven days. The potential therapeutic value of applying grafts of transfected fibroblasts expressing NGF in the model of retrograde atrophy of cholinergic neurons of the nucleus basalis magnocellularis (NBM) was assessed following partial devascularizing lesions of the cerebral cortex. We observed an increase in ChAT activity in the remaining cortex and a partial protection of the ipsilateral NBM, as determined by morphometric and biochemical studies.

Original languageEnglish
Pages (from-to)353-6
Number of pages4
JournalNeuroreport
Volume3
Issue number4
Publication statusPublished - Apr 1992

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Basal Ganglia
  • Cell Line
  • Cerebral Cortex
  • Choline O-Acetyltransferase
  • Fibroblasts
  • Male
  • Nerve Growth Factors
  • Neurons
  • Rats
  • Rats, Inbred Strains
  • Regional Blood Flow
  • Transfection

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