Reduced evolutionary rate in reemerged Ebola virus transmission chains

David J Blackley; Michael R Wiley; Jason T Ladner; Mosoka Fallah; Terrence Lo; Merle L Gilbert; Christopher Gregory; Jonathan D'ambrozio; Stewart Coulter; Suzanne Mate; Zephaniah Balogun; Jeffrey Kugelman; William Nwachukwu; Karla Prieto; Adolphus Yeiah; Fred Amegashie; Brian Kearney; Meagan Wisniewski; John Saindon; Gary Schroth; Lawrence Fakoli; Joseph W Diclaro; Jens H Kuhn; Lisa E Hensley; Peter B Jahrling; Ute Ströher; Stuart T Nichol; Moses Massaquoi; Francis Kateh; Peter Clement; Alex Gasasira; Fatorma Bolay; Stephan S Monroe; Andrew Rambaut; Mariano Sanchez-Lockhart; A Scott Laney; Tolbert Nyenswah; Athalia Christie; Gustavo Palacios

doi:10.1126/sciadv.1600378

Reduced evolutionary rate in reemerged Ebola virus transmission chains

David J Blackley, Michael R Wiley, Jason T Ladner, Mosoka Fallah, Terrence Lo, Merle L Gilbert, Christopher Gregory, Jonathan D'ambrozio, Stewart Coulter, Suzanne Mate, Zephaniah Balogun, Jeffrey Kugelman, William Nwachukwu, Karla Prieto, Adolphus Yeiah, Fred Amegashie, Brian Kearney, Meagan Wisniewski, John Saindon, Gary SchrothLawrence Fakoli, Joseph W Diclaro, Jens H Kuhn, Lisa E Hensley, Peter B Jahrling, Ute Ströher, Stuart T Nichol, Moses Massaquoi, Francis Kateh, Peter Clement, Alex Gasasira, Fatorma Bolay, Stephan S Monroe, Andrew Rambaut, Mariano Sanchez-Lockhart, A Scott Laney, Tolbert Nyenswah, Athalia Christie, Gustavo Palacios

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

On 29 June 2015, Liberia's respite from Ebola virus disease (EVD) was interrupted for the second time by a renewed outbreak ("flare-up") of seven confirmed cases. We demonstrate that, similar to the March 2015 flare-up associated with sexual transmission, this new flare-up was a reemergence of a Liberian transmission chain originating from a persistently infected source rather than a reintroduction from a reservoir or a neighboring country with active transmission. Although distinct, Ebola virus (EBOV) genomes from both flare-ups exhibit significantly low genetic divergence, indicating a reduced rate of EBOV evolution during persistent infection. Using this rate of change as a signature, we identified two additional EVD clusters that possibly arose from persistently infected sources. These findings highlight the risk of EVD flare-ups even after an outbreak is declared over.

Original language	English
Pages (from-to)	e1600378
Journal	Science Advances
Volume	2
Issue number	4
DOIs	https://doi.org/10.1126/sciadv.1600378
Publication status	Published - 29 Apr 2016

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e1600378.full
2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1600378
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Blackley, D. J., Wiley, M. R., Ladner, J. T., Fallah, M., Lo, T., Gilbert, M. L., Gregory, C., D'ambrozio, J., Coulter, S., Mate, S., Balogun, Z., Kugelman, J., Nwachukwu, W., Prieto, K., Yeiah, A., Amegashie, F., Kearney, B., Wisniewski, M., Saindon, J., ... Palacios, G. (2016). Reduced evolutionary rate in reemerged Ebola virus transmission chains. Science Advances, 2(4), e1600378. https://doi.org/10.1126/sciadv.1600378

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title = "Reduced evolutionary rate in reemerged Ebola virus transmission chains",

abstract = "On 29 June 2015, Liberia's respite from Ebola virus disease (EVD) was interrupted for the second time by a renewed outbreak ({"}flare-up{"}) of seven confirmed cases. We demonstrate that, similar to the March 2015 flare-up associated with sexual transmission, this new flare-up was a reemergence of a Liberian transmission chain originating from a persistently infected source rather than a reintroduction from a reservoir or a neighboring country with active transmission. Although distinct, Ebola virus (EBOV) genomes from both flare-ups exhibit significantly low genetic divergence, indicating a reduced rate of EBOV evolution during persistent infection. Using this rate of change as a signature, we identified two additional EVD clusters that possibly arose from persistently infected sources. These findings highlight the risk of EVD flare-ups even after an outbreak is declared over.",

author = "Blackley, \{David J\} and Wiley, \{Michael R\} and Ladner, \{Jason T\} and Mosoka Fallah and Terrence Lo and Gilbert, \{Merle L\} and Christopher Gregory and Jonathan D'ambrozio and Stewart Coulter and Suzanne Mate and Zephaniah Balogun and Jeffrey Kugelman and William Nwachukwu and Karla Prieto and Adolphus Yeiah and Fred Amegashie and Brian Kearney and Meagan Wisniewski and John Saindon and Gary Schroth and Lawrence Fakoli and Diclaro, \{Joseph W\} and Kuhn, \{Jens H\} and Hensley, \{Lisa E\} and Jahrling, \{Peter B\} and Ute Str{\"o}her and Nichol, \{Stuart T\} and Moses Massaquoi and Francis Kateh and Peter Clement and Alex Gasasira and Fatorma Bolay and Monroe, \{Stephan S\} and Andrew Rambaut and Mariano Sanchez-Lockhart and \{Scott Laney\}, A and Tolbert Nyenswah and Athalia Christie and Gustavo Palacios",

note = "OPen Access journal 2016 {\textcopyright} The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1600378 Claire Conlon 26/01/2017",

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Blackley, DJ, Wiley, MR, Ladner, JT, Fallah, M, Lo, T, Gilbert, ML, Gregory, C, D'ambrozio, J, Coulter, S, Mate, S, Balogun, Z, Kugelman, J, Nwachukwu, W, Prieto, K, Yeiah, A, Amegashie, F, Kearney, B, Wisniewski, M, Saindon, J, Schroth, G, Fakoli, L, Diclaro, JW, Kuhn, JH, Hensley, LE, Jahrling, PB, Ströher, U, Nichol, ST, Massaquoi, M, Kateh, F, Clement, P, Gasasira, A, Bolay, F, Monroe, SS, Rambaut, A, Sanchez-Lockhart, M, Scott Laney, A, Nyenswah, T, Christie, A & Palacios, G 2016, 'Reduced evolutionary rate in reemerged Ebola virus transmission chains', Science Advances, vol. 2, no. 4, pp. e1600378. https://doi.org/10.1126/sciadv.1600378

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T1 - Reduced evolutionary rate in reemerged Ebola virus transmission chains

AU - Blackley, David J

AU - Wiley, Michael R

AU - Ladner, Jason T

AU - Fallah, Mosoka

AU - Lo, Terrence

AU - Gilbert, Merle L

AU - Gregory, Christopher

AU - D'ambrozio, Jonathan

AU - Coulter, Stewart

AU - Mate, Suzanne

AU - Balogun, Zephaniah

AU - Kugelman, Jeffrey

AU - Nwachukwu, William

AU - Prieto, Karla

AU - Yeiah, Adolphus

AU - Amegashie, Fred

AU - Kearney, Brian

AU - Wisniewski, Meagan

AU - Saindon, John

AU - Schroth, Gary

AU - Fakoli, Lawrence

AU - Diclaro, Joseph W

AU - Kuhn, Jens H

AU - Hensley, Lisa E

AU - Jahrling, Peter B

AU - Ströher, Ute

AU - Nichol, Stuart T

AU - Massaquoi, Moses

AU - Kateh, Francis

AU - Clement, Peter

AU - Gasasira, Alex

AU - Bolay, Fatorma

AU - Monroe, Stephan S

AU - Rambaut, Andrew

AU - Sanchez-Lockhart, Mariano

AU - Scott Laney, A

AU - Nyenswah, Tolbert

AU - Christie, Athalia

AU - Palacios, Gustavo

N1 - OPen Access journal 2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1600378 Claire Conlon 26/01/2017

PY - 2016/4/29

Y1 - 2016/4/29

N2 - On 29 June 2015, Liberia's respite from Ebola virus disease (EVD) was interrupted for the second time by a renewed outbreak ("flare-up") of seven confirmed cases. We demonstrate that, similar to the March 2015 flare-up associated with sexual transmission, this new flare-up was a reemergence of a Liberian transmission chain originating from a persistently infected source rather than a reintroduction from a reservoir or a neighboring country with active transmission. Although distinct, Ebola virus (EBOV) genomes from both flare-ups exhibit significantly low genetic divergence, indicating a reduced rate of EBOV evolution during persistent infection. Using this rate of change as a signature, we identified two additional EVD clusters that possibly arose from persistently infected sources. These findings highlight the risk of EVD flare-ups even after an outbreak is declared over.

AB - On 29 June 2015, Liberia's respite from Ebola virus disease (EVD) was interrupted for the second time by a renewed outbreak ("flare-up") of seven confirmed cases. We demonstrate that, similar to the March 2015 flare-up associated with sexual transmission, this new flare-up was a reemergence of a Liberian transmission chain originating from a persistently infected source rather than a reintroduction from a reservoir or a neighboring country with active transmission. Although distinct, Ebola virus (EBOV) genomes from both flare-ups exhibit significantly low genetic divergence, indicating a reduced rate of EBOV evolution during persistent infection. Using this rate of change as a signature, we identified two additional EVD clusters that possibly arose from persistently infected sources. These findings highlight the risk of EVD flare-ups even after an outbreak is declared over.

U2 - 10.1126/sciadv.1600378

DO - 10.1126/sciadv.1600378

M3 - Article

C2 - 27386513

SN - 2375-2548

VL - 2

SP - e1600378

JO - Science Advances

JF - Science Advances

IS - 4

ER -

Reduced evolutionary rate in reemerged Ebola virus transmission chains

Abstract

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