Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation

Pawan Kumar Jewaria; Bibek Aryal; Rifat Ara Begum; Yaowei Wang; Gloria Sancho-Andrés; Abu Imran  Baba; Meng Yu; Xiaojuan Li; Jinxing Lin; Stephen C. Fry; Stephane Verger; Eugenia Russinova; Kristoffer Jonsson; Rishikesh P. Bhalerao

doi:10.1126/sciadv.ads0760

Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation

Pawan Kumar Jewaria, Bibek Aryal, Rifat Ara Begum, Yaowei Wang, Gloria Sancho-Andrés , Abu Imran Baba, Meng Yu, Xiaojuan Li, Jinxing Lin, Stephen C. Fry, Stephane Verger, Eugenia Russinova, Kristoffer Jonsson, Rishikesh P. Bhalerao^*

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Defects in cell wall integrity (CWI) profoundly affect plant growth, although, underlying mechanisms are not well understood. We show that in Arabidopsis mur1 mutant, CWI defects from compromising dimerization of RG-II pectin, a key component of cell wall, attenuate the expression of auxin response factors ARF7-ARF19. As a result, polar auxin transport components are misexpressed, disrupting auxin response asymmetry, leading to defective apical hook development. Accordingly, mur1 hook defects are suppressed by enhancing ARF7 expression. In addition, expression of brassinosteroid biosynthesis genes is down-regulated in mur1 mutant, and supplementing brassinosteroid or enhancing brassinosteroid signaling suppresses mur1 hook defects. Intriguingly, brassinosteroid enhances RG-II dimerization, showing hormonal feedback to the cell wall. Our results thus reveal a previously unrecognized link between cell wall defects from reduced RG-II dimerization and growth regulation mediated via modulation of auxin-brassinosteroid pathways in early seedling development.

Original language	English
Article number	eads0760
Number of pages	10
Journal	Science Advances
Volume	11
Issue number	7
Early online date	12 Feb 2025
DOIs	https://doi.org/10.1126/sciadv.ads0760
Publication status	Published - 14 Feb 2025

Keywords / Materials (for Non-textual outputs)

Cell wall
Auxin
Brassinosteroid
Arabidopsis
Apical hook

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title = "Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation",

abstract = "Defects in cell wall integrity (CWI) profoundly affect plant growth, although, underlying mechanisms are not well understood. We show that in Arabidopsis mur1 mutant, CWI defects from compromising dimerization of RG-II pectin, a key component of cell wall, attenuate the expression of auxin response factors ARF7-ARF19. As a result, polar auxin transport components are misexpressed, disrupting auxin response asymmetry, leading to defective apical hook development. Accordingly, mur1 hook defects are suppressed by enhancing ARF7 expression. In addition, expression of brassinosteroid biosynthesis genes is down-regulated in mur1 mutant, and supplementing brassinosteroid or enhancing brassinosteroid signaling suppresses mur1 hook defects. Intriguingly, brassinosteroid enhances RG-II dimerization, showing hormonal feedback to the cell wall. Our results thus reveal a previously unrecognized link between cell wall defects from reduced RG-II dimerization and growth regulation mediated via modulation of auxin-brassinosteroid pathways in early seedling development. ",

keywords = "Cell wall, Auxin, Brassinosteroid, Arabidopsis, Apical hook",

author = "Jewaria, \{Pawan Kumar\} and Bibek Aryal and Begum, \{Rifat Ara\} and Yaowei Wang and Gloria Sancho-Andr{\'e}s and Baba, \{Abu Imran\} and Meng Yu and Xiaojuan Li and Jinxing Lin and Fry, \{Stephen C.\} and Stephane Verger and Eugenia Russinova and Kristoffer Jonsson and Bhalerao, \{Rishikesh P.\}",

note = "We thank Prof. Sigal Savaldi-Goldstein for helpful comments on the manuscript. We thank Y. Yin for providing the anti-BES1 antibody. The authors acknowledge the facilities and technical assistance of the Ume{\aa} Plant Science Centre Microscopy facility and the plant growth facility. ",

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doi = "10.1126/sciadv.ads0760",

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T1 - Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation

AU - Jewaria, Pawan Kumar

AU - Aryal, Bibek

AU - Begum, Rifat Ara

AU - Wang, Yaowei

AU - Sancho-Andrés , Gloria

AU - Baba, Abu Imran

AU - Yu, Meng

AU - Li, Xiaojuan

AU - Lin, Jinxing

AU - Fry, Stephen C.

AU - Verger, Stephane

AU - Russinova, Eugenia

AU - Jonsson, Kristoffer

AU - Bhalerao, Rishikesh P.

N1 - We thank Prof. Sigal Savaldi-Goldstein for helpful comments on the manuscript. We thank Y. Yin for providing the anti-BES1 antibody. The authors acknowledge the facilities and technical assistance of the Umeå Plant Science Centre Microscopy facility and the plant growth facility.

PY - 2025/2/14

Y1 - 2025/2/14

N2 - Defects in cell wall integrity (CWI) profoundly affect plant growth, although, underlying mechanisms are not well understood. We show that in Arabidopsis mur1 mutant, CWI defects from compromising dimerization of RG-II pectin, a key component of cell wall, attenuate the expression of auxin response factors ARF7-ARF19. As a result, polar auxin transport components are misexpressed, disrupting auxin response asymmetry, leading to defective apical hook development. Accordingly, mur1 hook defects are suppressed by enhancing ARF7 expression. In addition, expression of brassinosteroid biosynthesis genes is down-regulated in mur1 mutant, and supplementing brassinosteroid or enhancing brassinosteroid signaling suppresses mur1 hook defects. Intriguingly, brassinosteroid enhances RG-II dimerization, showing hormonal feedback to the cell wall. Our results thus reveal a previously unrecognized link between cell wall defects from reduced RG-II dimerization and growth regulation mediated via modulation of auxin-brassinosteroid pathways in early seedling development.

AB - Defects in cell wall integrity (CWI) profoundly affect plant growth, although, underlying mechanisms are not well understood. We show that in Arabidopsis mur1 mutant, CWI defects from compromising dimerization of RG-II pectin, a key component of cell wall, attenuate the expression of auxin response factors ARF7-ARF19. As a result, polar auxin transport components are misexpressed, disrupting auxin response asymmetry, leading to defective apical hook development. Accordingly, mur1 hook defects are suppressed by enhancing ARF7 expression. In addition, expression of brassinosteroid biosynthesis genes is down-regulated in mur1 mutant, and supplementing brassinosteroid or enhancing brassinosteroid signaling suppresses mur1 hook defects. Intriguingly, brassinosteroid enhances RG-II dimerization, showing hormonal feedback to the cell wall. Our results thus reveal a previously unrecognized link between cell wall defects from reduced RG-II dimerization and growth regulation mediated via modulation of auxin-brassinosteroid pathways in early seedling development.

KW - Cell wall

KW - Auxin

KW - Brassinosteroid

KW - Arabidopsis

KW - Apical hook

U2 - 10.1126/sciadv.ads0760

DO - 10.1126/sciadv.ads0760

M3 - Article

C2 - 39937898

SN - 2375-2548

VL - 11

JO - Science Advances

JF - Science Advances

IS - 7

M1 - eads0760

ER -

Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation

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Keywords / Materials (for Non-textual outputs)

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