Reference-free SNP discovery for the Eurasian beaver from restriction site-associated DNA paired-end data

H. Senn, Rob Ogden, R. Mcewing, T. Cezard, K. Gharbi, Z. Iqbal, E. Johnson, N. Kamps-Hughes, F. Rosell

Research output: Contribution to journalSpecial issuepeer-review

Abstract

In this study, we used restriction site-associated DNA (RAD) sequencing to discover SNP markers suitable for population genetic and parentage analysis with the aim of using them for monitoring the reintroduction of the Eurasian beaver (Castor fibre) to Scotland. In the absence of a reference genome for beaver, we built contigs and discovered SNPs within them using paired-end RAD data, so as to have sufficient flanking region around the SNPs to conduct marker design. To do this, we used a simple pipeline which catalogued the Read 1 data in stacks and then used the assembler cortex_var to conduct de novo assembly and genotyping of multiple samples using the Read 2 data. The analysis of around 1.1 billion short reads of sequence data was reduced to a set of 2579 high-quality candidate SNP markers that were polymorphic in Norwegian and Bavarian beaver. Both laboratory validation of a subset of eight of the SNPs (1.3% error) and internal validation by confirming patterns of Mendelian inheritance in a family group (0.9% error) confirmed the success of this approach.
Original languageEnglish
JournalMolecular Ecology
Volume22
Issue number11
Early online date21 Feb 2013
DOIs
Publication statusPublished - 30 Jun 2013

Keywords

  • Castor fibre
  • conservation genetics
  • conservation genomics
  • genotyping by sequencing
  • reintroduction

Fingerprint

Dive into the research topics of 'Reference-free SNP discovery for the Eurasian beaver from restriction site-associated DNA paired-end data'. Together they form a unique fingerprint.

Cite this