This conclusion links the various contributions in light of the introductory framework. In line with our framework, scepticism towards the EU has increased since 2004 across most of the EU regions (old and new) and state-centric approaches (regional influence mediated through the central executives) have become the dominant strategy for regional mobilization. Unmediated access through direct regional representation in Brussels remains an important side-strategy though, especially for sub-state nations and regions with the highest level of regional authority, as theoretically expected. Regional authority—more so than the difference between competitive versus cooperative multi-level designs—is an important predictor against centralization pressures resulting from European integration. Overall, changes in the ‘New Europe’ have intensified but not transformed the dominant patterns of regional mobilization, while system-level and regional variables mediate impacts of Europeanization.