Regulation of asymmetrical cytokinesis by cAMP during meiosis I in mouse oocytes

Dawei Chen, Yuanwei Zhang, Qiyi Yi, Yun Huang, Heli Hou, Yingyin Zhang, Qiaomei Hao, Howard J Cooke, Lei Li, Qingyuan Sun, Qinghua Shi

Research output: Contribution to journalArticlepeer-review

Abstract

Mammalian oocytes undergo an asymmetrical first meiotic division, extruding half of their chromosomes in a small polar body to preserve maternal resources for embryonic development. To divide asymmetrically, mammalian oocytes relocate chromosomes from the center of the cell to the cortex, but little is known about the underlying mechanisms. Here, we show that upon the elevation of intracellular cAMP level, mouse oocytes produced two daughter cells with similar sizes. This symmetrical cell division could be rescued by the inhibition of PKA, a cAMP-dependent protein kinase. Live cell imaging revealed that a symmetrically localized cleavage furrow resulted in symmetrical cell division. Detailed analyses demonstrated that symmetrically localized cleavage furrows were caused by the inappropriate central positioning of chromosome clusters at anaphase onset, indicating that chromosome cluster migration was impaired. Notably, high intracellular cAMP reduced myosin II activity, and the microinjection of phospho-myosin II antibody into the oocytes impeded chromosome migration and promoted symmetrical cell division. Our results support the hypothesis that cAMP plays a role in regulating asymmetrical cell division by modulating myosin II activity during mouse oocyte meiosis I, providing a novel insight into the regulation of female gamete formation in mammals.
Original languageEnglish
Pages (from-to)e29735
JournalPLoS ONE
Volume7
Issue number1
DOIs
Publication statusPublished - 2012

Keywords

  • 1-Methyl-3-isobutylxanthine
  • Anaphase
  • Animals
  • Asymmetric Cell Division
  • Bucladesine
  • Cell Movement
  • Cells, Cultured
  • Chromosomes, Mammalian
  • Cyclic AMP
  • Cytokinesis
  • Female
  • Intracellular Space
  • Isoquinolines
  • Meiosis
  • Mice
  • Mice, Inbred ICR
  • Microinjections
  • Myosin Type II
  • Oocytes
  • Sulfonamides

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