Regulation of LPCAT3 by LXR

O Demeure; F Lecerf; C Duby; C Desert; S Ducheix; H Guillou; S Lagarrigue

doi:10.1016/j.gene.2010.09.002

Regulation of LPCAT3 by LXR

O Demeure, F Lecerf, C Duby, C Desert, S Ducheix, H Guillou, S Lagarrigue

Work enabled by Edinburgh Genomics

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In this work we analyzed the transcriptome profiles of chicken hepatoma cells (LMH) in response to T0901317, a pharmacological agonist of the liver X receptor (LXR). Through an in silico search for LXRE (LXR response element) consensus sequences in the promoter of genes whose expression was shown to be sensitive to TO901317, we identified a LXRE in the promoter of the LPCAT3 (lysophosphatidylcholine acyltransferase 3). This motif is highly conserved between species. We further investigated the regulation of this gene and showed that the expression of LPCAT3 was induced both in chicken and human hepatoma cells (LMH and HuH-7, respectively) in response to T0901317. Transactivation and electrophoretic mobility shift assays allowed us to locate a functional LXRE in the chicken LPCAT3 promoter. Altogether these data evidence for the first time that the chicken LPCAT3 gene is a direct target of LXR and therefore suggest a new role for LXR in phospholipid homeostasis.

Original language	English
Pages (from-to)	7-11
Number of pages	5
Journal	Gene
Volume	470
Issue number	1-2
DOIs	https://doi.org/10.1016/j.gene.2010.09.002
Publication status	Published - 1 Jan 2011

Keywords / Materials (for Non-textual outputs)

1-Acylglycerophosphocholine O-Acyltransferase
Animals
Cell Line, Tumor
Chickens
Fatty Acids
Gene Expression Profiling
Humans
Hydrocarbons, Fluorinated
Orphan Nuclear Receptors
Sulfonamides

Access to Document

10.1016/j.gene.2010.09.002

Ark Genomics
Watson, M., Archibald, A. & Burt, D.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

@article{9c50d6c3d8034551aa7a7ddb663218d6,

title = "Regulation of LPCAT3 by LXR",

abstract = "In this work we analyzed the transcriptome profiles of chicken hepatoma cells (LMH) in response to T0901317, a pharmacological agonist of the liver X receptor (LXR). Through an in silico search for LXRE (LXR response element) consensus sequences in the promoter of genes whose expression was shown to be sensitive to TO901317, we identified a LXRE in the promoter of the LPCAT3 (lysophosphatidylcholine acyltransferase 3). This motif is highly conserved between species. We further investigated the regulation of this gene and showed that the expression of LPCAT3 was induced both in chicken and human hepatoma cells (LMH and HuH-7, respectively) in response to T0901317. Transactivation and electrophoretic mobility shift assays allowed us to locate a functional LXRE in the chicken LPCAT3 promoter. Altogether these data evidence for the first time that the chicken LPCAT3 gene is a direct target of LXR and therefore suggest a new role for LXR in phospholipid homeostasis.",

keywords = "1-Acylglycerophosphocholine O-Acyltransferase, Animals, Cell Line, Tumor, Chickens, Fatty Acids, Gene Expression Profiling, Humans, Hydrocarbons, Fluorinated, Orphan Nuclear Receptors, Sulfonamides",

author = "O Demeure and F Lecerf and C Duby and C Desert and S Ducheix and H Guillou and S Lagarrigue",

year = "2011",

month = jan,

day = "1",

doi = "10.1016/j.gene.2010.09.002",

language = "English",

volume = "470",

pages = "7--11",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Regulation of LPCAT3 by LXR

AU - Demeure, O

AU - Lecerf, F

AU - Duby, C

AU - Desert, C

AU - Ducheix, S

AU - Guillou, H

AU - Lagarrigue, S

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In this work we analyzed the transcriptome profiles of chicken hepatoma cells (LMH) in response to T0901317, a pharmacological agonist of the liver X receptor (LXR). Through an in silico search for LXRE (LXR response element) consensus sequences in the promoter of genes whose expression was shown to be sensitive to TO901317, we identified a LXRE in the promoter of the LPCAT3 (lysophosphatidylcholine acyltransferase 3). This motif is highly conserved between species. We further investigated the regulation of this gene and showed that the expression of LPCAT3 was induced both in chicken and human hepatoma cells (LMH and HuH-7, respectively) in response to T0901317. Transactivation and electrophoretic mobility shift assays allowed us to locate a functional LXRE in the chicken LPCAT3 promoter. Altogether these data evidence for the first time that the chicken LPCAT3 gene is a direct target of LXR and therefore suggest a new role for LXR in phospholipid homeostasis.

AB - In this work we analyzed the transcriptome profiles of chicken hepatoma cells (LMH) in response to T0901317, a pharmacological agonist of the liver X receptor (LXR). Through an in silico search for LXRE (LXR response element) consensus sequences in the promoter of genes whose expression was shown to be sensitive to TO901317, we identified a LXRE in the promoter of the LPCAT3 (lysophosphatidylcholine acyltransferase 3). This motif is highly conserved between species. We further investigated the regulation of this gene and showed that the expression of LPCAT3 was induced both in chicken and human hepatoma cells (LMH and HuH-7, respectively) in response to T0901317. Transactivation and electrophoretic mobility shift assays allowed us to locate a functional LXRE in the chicken LPCAT3 promoter. Altogether these data evidence for the first time that the chicken LPCAT3 gene is a direct target of LXR and therefore suggest a new role for LXR in phospholipid homeostasis.

KW - 1-Acylglycerophosphocholine O-Acyltransferase

KW - Animals

KW - Cell Line, Tumor

KW - Chickens

KW - Fatty Acids

KW - Gene Expression Profiling

KW - Humans

KW - Hydrocarbons, Fluorinated

KW - Orphan Nuclear Receptors

KW - Sulfonamides

U2 - 10.1016/j.gene.2010.09.002

DO - 10.1016/j.gene.2010.09.002

M3 - Article

C2 - 20837115

SN - 0378-1119

VL - 470

SP - 7

EP - 11

JO - Gene

JF - Gene

IS - 1-2

ER -

Regulation of LPCAT3 by LXR

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

Ark Genomics

Cite this