Abstract / Description of output
We examined the activation of nNOS mRNA expression within the supraoptic and paraventricular nuclei (SON and PVN) of the hypothalamus. In salt-loaded rats nNOS mRNA expression was significantly increased in both nuclei. In rats given i.p. injections of 1.5 M NaCl (4 ml/kg), a small but significant increase in nNOS mRNA expression in the SON and PVN was found 6 h after injection; no change was detected 2 or 4 h after injection. In rats in which hyponatraemia had been induced experimentally, nNOS mRNA was downregulated in the SON, and expression levels were not increased within 4 h after intense acute osmotic stimuli. Finally, neurons of the SON were antidromically-activated by neural stalk stimulation for 2 h. No increase of nNOS mRNA expression in the SON was observed 2 h after stimulation. Thus, increased electrical activity is not directly coupled to rapidly increased expression of nNOS mRNA, and hence acute increases in nNOS mRNA expression are unlikely to play a role in short-term adaptation of the magnocellular system to osmotic stimulation.
Original language | English |
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Pages (from-to) | 191-6 |
Number of pages | 6 |
Journal | Neuroscience Letters |
Volume | 369 |
Issue number | 3 |
DOIs | |
Publication status | Published - 21 Oct 2004 |
Keywords / Materials (for Non-textual outputs)
- Analysis of Variance
- Animals
- Cell Count/methods
- Dose-Response Relationship, Drug
- Female
- Gene Expression Regulation/drug effects
- Gene Expression Regulation/physiology
- In Situ Hybridization/methods
- Neurosecretory Systems/drug effects
- Neurosecretory Systems/metabolism
- Nitric Oxide Synthase/genetics
- Nitric Oxide Synthase/metabolism
- Nitric Oxide Synthase Type I
- Paraventricular Hypothalamic Nucleus/cytology
- Paraventricular Hypothalamic Nucleus/metabolism
- RNA, Messenger/metabolism
- Rats
- Rats, Sprague-Dawley
- Saline Solution, Hypertonic/pharmacology
- Supraoptic Nucleus/cytology
- Supraoptic Nucleus/metabolism
- Time Factors