Regulation of Ov2 by virus encoded microRNAs

Katie Nightingale; Inga Dry; John Hopkins; Robert Dalziel

doi:10.1007/s11259-019-09749-9

Regulation of Ov2 by virus encoded microRNAs

Katie Nightingale, Inga Dry, John Hopkins, Robert Dalziel

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Herpesviruses encode miRNAs that target both virus and host genes; however their role in herpesvirus biology is still poorly understood. We previously identified thirty five miRNAs encoded by OvHV-2; the causative agent of malignant catarrhal fever (MCF) and are investigating the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. Analysis, using RNAHybrid predicted that two OvHV-2
encoded miRNAs, ovhv2-miR-17-10 and ovhv2-miR-61-1, target transcripts coding for the OvHV-2 bZIP protein Ov2. In other herpesvirus bZIP proteins are known to play important roles in lytic virus replication.
Here we show by Flow cytometry and western blotting that ovhv2-miR-17-10 and ovhv2- miR-61-1, reduce the expression of Ov2 protein. The predicted target sites for both miRNAs within the Ov2 gene were disrupted whilst retaining the Ov2 coding sequence. Mutation of the ovhv2-miR-61-1 target sequence restored Ov2 protein expression levels to control levels
confirming the identity of its target site. However, it was not possible to determine the binding site of ovhv2-miR-17-10 possibly due to potential G:U pairing introduced during the mutation process.

Original language	English
Journal	Veterinary Research Communications
Early online date	19 Mar 2019
DOIs	https://doi.org/10.1007/s11259-019-09749-9
Publication status	E-pub ahead of print - 19 Mar 2019

Keywords

Ovine herpesvirus-2
Virus encoded miRNAs, Ov2

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10.1007/s11259-019-09749-9

Regulation of Ov2 by virus encoded microRNAs
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title = "Regulation of Ov2 by virus encoded microRNAs",

abstract = "Herpesviruses encode miRNAs that target both virus and host genes; however their role in herpesvirus biology is still poorly understood. We previously identified thirty five miRNAs encoded by OvHV-2; the causative agent of malignant catarrhal fever (MCF) and are investigating the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. Analysis, using RNAHybrid predicted that two OvHV-2encoded miRNAs, ovhv2-miR-17-10 and ovhv2-miR-61-1, target transcripts coding for the OvHV-2 bZIP protein Ov2. In other herpesvirus bZIP proteins are known to play important roles in lytic virus replication.Here we show by Flow cytometry and western blotting that ovhv2-miR-17-10 and ovhv2- miR-61-1, reduce the expression of Ov2 protein. The predicted target sites for both miRNAs within the Ov2 gene were disrupted whilst retaining the Ov2 coding sequence. Mutation of the ovhv2-miR-61-1 target sequence restored Ov2 protein expression levels to control levelsconfirming the identity of its target site. However, it was not possible to determine the binding site of ovhv2-miR-17-10 possibly due to potential G:U pairing introduced during the mutation process.",

keywords = "Ovine herpesvirus-2, Virus encoded miRNAs, Ov2",

author = "Katie Nightingale and Inga Dry and John Hopkins and Robert Dalziel",

year = "2019",

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doi = "10.1007/s11259-019-09749-9",

language = "English",

journal = "Veterinary Research Communications",

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TY - JOUR

T1 - Regulation of Ov2 by virus encoded microRNAs

AU - Nightingale, Katie

AU - Dry, Inga

AU - Hopkins, John

AU - Dalziel, Robert

PY - 2019/3/19

Y1 - 2019/3/19

N2 - Herpesviruses encode miRNAs that target both virus and host genes; however their role in herpesvirus biology is still poorly understood. We previously identified thirty five miRNAs encoded by OvHV-2; the causative agent of malignant catarrhal fever (MCF) and are investigating the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. Analysis, using RNAHybrid predicted that two OvHV-2encoded miRNAs, ovhv2-miR-17-10 and ovhv2-miR-61-1, target transcripts coding for the OvHV-2 bZIP protein Ov2. In other herpesvirus bZIP proteins are known to play important roles in lytic virus replication.Here we show by Flow cytometry and western blotting that ovhv2-miR-17-10 and ovhv2- miR-61-1, reduce the expression of Ov2 protein. The predicted target sites for both miRNAs within the Ov2 gene were disrupted whilst retaining the Ov2 coding sequence. Mutation of the ovhv2-miR-61-1 target sequence restored Ov2 protein expression levels to control levelsconfirming the identity of its target site. However, it was not possible to determine the binding site of ovhv2-miR-17-10 possibly due to potential G:U pairing introduced during the mutation process.

AB - Herpesviruses encode miRNAs that target both virus and host genes; however their role in herpesvirus biology is still poorly understood. We previously identified thirty five miRNAs encoded by OvHV-2; the causative agent of malignant catarrhal fever (MCF) and are investigating the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. Analysis, using RNAHybrid predicted that two OvHV-2encoded miRNAs, ovhv2-miR-17-10 and ovhv2-miR-61-1, target transcripts coding for the OvHV-2 bZIP protein Ov2. In other herpesvirus bZIP proteins are known to play important roles in lytic virus replication.Here we show by Flow cytometry and western blotting that ovhv2-miR-17-10 and ovhv2- miR-61-1, reduce the expression of Ov2 protein. The predicted target sites for both miRNAs within the Ov2 gene were disrupted whilst retaining the Ov2 coding sequence. Mutation of the ovhv2-miR-61-1 target sequence restored Ov2 protein expression levels to control levelsconfirming the identity of its target site. However, it was not possible to determine the binding site of ovhv2-miR-17-10 possibly due to potential G:U pairing introduced during the mutation process.

KW - Ovine herpesvirus-2

KW - Virus encoded miRNAs, Ov2

U2 - 10.1007/s11259-019-09749-9

DO - 10.1007/s11259-019-09749-9

M3 - Article

JO - Veterinary Research Communications

JF - Veterinary Research Communications

SN - 0165-7380

ER -

Regulation of Ov2 by virus encoded microRNAs

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