Regulation of Ov2 by virus encoded microRNAs

Katie Nightingale, Inga Dry, John Hopkins, Robert Dalziel

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Herpesviruses encode miRNAs that target both virus and host genes; however their role in herpesvirus biology is still poorly understood. We previously identified thirty five miRNAs encoded by OvHV-2; the causative agent of malignant catarrhal fever (MCF) and are investigating the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. Analysis, using RNAHybrid predicted that two OvHV-2
encoded miRNAs, ovhv2-miR-17-10 and ovhv2-miR-61-1, target transcripts coding for the OvHV-2 bZIP protein Ov2. In other herpesvirus bZIP proteins are known to play important roles in lytic virus replication.
Here we show by Flow cytometry and western blotting that ovhv2-miR-17-10 and ovhv2- miR-61-1, reduce the expression of Ov2 protein. The predicted target sites for both miRNAs within the Ov2 gene were disrupted whilst retaining the Ov2 coding sequence. Mutation of the ovhv2-miR-61-1 target sequence restored Ov2 protein expression levels to control levels
confirming the identity of its target site. However, it was not possible to determine the binding site of ovhv2-miR-17-10 possibly due to potential G:U pairing introduced during the mutation process.
Original languageEnglish
JournalVeterinary Research Communications
Early online date19 Mar 2019
DOIs
Publication statusE-pub ahead of print - 19 Mar 2019

Keywords / Materials (for Non-textual outputs)

  • Ovine herpesvirus-2
  • Virus encoded miRNAs, Ov2

Fingerprint

Dive into the research topics of 'Regulation of Ov2 by virus encoded microRNAs'. Together they form a unique fingerprint.

Cite this